Protein stability analysis in ionic liquids by 19F NMR

被引：15

作者：

Cheng, Kai ^{[1
]}

Wu, Qiong ^{[1
]}

Jiang, Ling ^{[1
]}

Liu, Maili ^{[1
]}

Li, Conggang ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math,Natl Ctr Magnet Resonance, Wuhan 430071, Hubei, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2019年 / 411卷 / 19期

关键词：

Ionic liquids; Protein stability quantification; F-19; NMR; ANTARCTICA LIPASE-B; THERMAL-STABILITY; HOFMEISTER SERIES; CYTOCHROME-C; STABILIZATION; EXTRACTION; INTERMEDIATE; DISSOLUTION; IMPROVEMENT; HEMOGLOBIN;

D O I：

10.1007/s00216-019-01804-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ionic liquids have been extensively used as environmentally friendly solvents for enzymatic reactions and other biological systems. Understanding the mechanism of how ionic liquids affect protein stability is crucial for the biological reaction processes and protein storage using ionic liquids as solvents. Although effects of ionic liquids on protein stability have been studied, equilibrium thermodynamics of protein stability in ionic liquids has not been quantitatively measured. Herein, we utilized F-19 NMR to measure the equilibrium thermodynamics of protein stability in ionic liquid [C-4-mim]Br. Our results show that proteins are significantly destabilized in [C-4-mim]Br ionic liquids. Our results suggest that F-19 NMR provides a simple and effective way to study the thermodynamics of protein stability in ionic liquids. F-19 NMR will be applicable to facilitate the protein-protein interaction study in ionic liquids.

引用

页码：4929 / 4935

页数：7

共 62 条

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