Pituitary Adenylate Cyclase-activating Polypeptide-like Compounds Could Modulate the Activity of Coelomocytes in the Earthworm

被引：9

作者：

Somogyi, Ildiko ^{[2
]}

Boros, Akos ^{[2
]}

Engelmann, Peter ^{[3
]}

Varhalmi, Eszter ^{[2
]}

Nemeth, Jozsef ^{[4
]}

Lubics, Andrea ^{[1
]}

Tamas, Andrea ^{[1
]}

Kiss, Peter ^{[1
]}

Reglodi, Dora ^{[1
]}

Pollak, Edit ^{[2
]}

Molnar, Laszlo ^{[2
]}

机构：

[1] Univ Pecs, Dept Anat, H-7624 Pecs, Hungary

[2] Univ Pecs, Dept Gen Zool, H-7624 Pecs, Hungary

[3] Univ Pecs, Dept Immunol & Biotechnol, H-7624 Pecs, Hungary

[4] Univ Debrecen, Dept Pharmacol & Pharmacotherapy, H-4012 Debrecen, Hungary

来源：

TRENDS IN COMPARATIVE ENDOCRINOLOGY AND NEUROBIOLOGY | 2009年 / 1163卷

关键词：

radioimmunoassay; Western blot; immunocytochemistry; Eisenia fetida; EXPRESSION; PACAP;

D O I：

10.1111/j.1749-6632.2009.04431.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

By means of radioimmunoassay, we studied the concentration of pituitary adenylate cyclase-activating polypeptide (PACAP)-like proteins in intact and regenerating earthworms. Transection of animals increased the concentration of PACAP-like compounds in coelomocytes, and a decreasing rostrocaudal gradient was detected in the regenerating animals. Western blot analysis revealed a range of PAC1-receptor proteins with molecular weights from 40 to 80 kDa. Electron microscopic immunocytochemistry showed that PAC1 receptors were located on distinct sets of coelomocytes (mainly on amebocytes and on some granulocytes). Based on our results we hypothesize a link between PACAP and coelomocytes, suggesting that PACAP modulates the function of amebocytes and certain granulocytes that play a role in tissue remodeling of regenerating earthworms.

引用

页码：521 / 523

页数：3

共 6 条

[1] Pituitary adenylyl cyclase-activating polypeptide (PACAP) and its receptor (PAC1-R) in the cochlea: Evidence for specific transcript expression of PAC1-R splice variants in rat microdissected cochlear subfractions
Abu-Hamdan, M. D.
Drescher, M. J.
Ramakrishnan, N. A.
Khan, K. M.
Toma, V. S.
Hatfield, J. S.
Drescher, D. G.
[J]. NEUROSCIENCE, 2006, 140 (01) : 147 - 161
[2] Cooper E.L., 1996, Progress in Molecular and Subcellular Biology, V15, P10
[3] THE MYELOERYTHROID NATURE OF THE CHLORAGOGENOUS-LIKE TISSUES OF THE ANNELIDS
FISCHER, E
[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1993, 106 (03): : 449 - 453
[4] JAMIESON BGM, 1981, ULTRASTRUCTURE OLIGO, P62
[5] PAC1 receptor localization in a model nervous system:: Light and electron microscopic immunocytochemistry on the earthworm ventral nerve cord ganglia
Molnar, L.
Pollak, E.
Boros, A.
Shioda, S.
Nakajo, S.
Tamas, A.
Lengvari, I.
Regloedi, D.
Lubics, A.
[J]. REGULATORY PEPTIDES, 2008, 145 (1-3) : 96 - 104
[6] Expression of PACAP-Like Compounds During the Caudal Regeneration of the Earthworm Eisenia fetida
Varhalmi, Eszter
Somogyi, Ildiko
Kiszler, Gabor
Nemeth, Jozsef
Reglodi, Dora
Lubics, Andrea
Kiss, Peter
Tamas, Andrea
Pollak, Edit
Molnar, Laszlo
[J]. JOURNAL OF MOLECULAR NEUROSCIENCE, 2008, 36 (1-3) : 166 - 174

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