Lactobacillus hulanensis sp. nov., isolated from suancai, a traditional Chinese pickle

被引:8
作者
Zhao, Wei [1 ]
Gu, Chun Tao [1 ]
机构
[1] Northeast Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Lactobacillus; novel species; pickle; lactic acid bacteria; MICROBIAL GENOMES; SEQUENCE-ANALYSIS; IDENTIFICATION; PHES; RPOA;
D O I
10.1099/ijsem.0.003453
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Gram-stain-positive bacterial strain, ZW163(T), isolated from suancai, a traditional pickle of Heilongjiang province in China, was characterized by a polyphasic approach, including phenotypic characterization, sequence analyses of the 16S rRNA gene and two housekeeping genes (pheS and rpoA), whole genome sequencing and cellular fatty acid composition. Strain ZW163(T) was phylogenetically related to Lactobacillus sharpeae, Lactobacillus songhuajiangensis, Lactobacillus pantheris and Lactobacillus thailandensis, having 93.8-98.7 % 16S rRNA gene sequence similarities, 76.5-87.1 % pheS gene sequence similarities and 74.3-95.7 % rpoA gene sequence similarities to the type strains of L. sharpeae, L. songhuajiangensis, L. pantheris and L. thailandensis, respectively. Average nucleotide identity and in silico DNA-DNA hybridization values between strain ZW163(T) and the type strains of L. sharpeae, L. songhuajiangensis, L. pantheris and L. thailandensis were less than 77.7 % and 23.6 % respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus hulanensis sp. nov., is proposed and the type strain is ZW163(T) (=NCIMB 15193(T)=CCM 8926(T)=CCTCC AB 2019015(T)).
引用
收藏
页码:2147 / 2152
页数:6
相关论文
共 27 条
[1]   Actinomyces ruminicola sp nov., isolated from cattle rumen [J].
An, Dengdi ;
Cai, Shichun ;
Dong, Xiuzhu .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 :2043-2048
[2]   GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions [J].
Besemer, J ;
Lomsadze, A ;
Borodovsky, M .
NUCLEIC ACIDS RESEARCH, 2001, 29 (12) :2607-2618
[3]   A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data [J].
Coil, David ;
Jospin, Guillaume ;
Darling, Aaron E. .
BIOINFORMATICS, 2015, 31 (04) :587-589
[4]   DNA-DNA hybridization values and their relationship to whole-genome sequence similarities [J].
Goris, Johan ;
Konstantinidis, Konstantinos T. ;
Klappenbach, Joel A. ;
Coenye, Tom ;
Vandamme, Peter ;
Tiedje, James M. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007, 57 :81-91
[5]   Lactobacillus mudanjiangensis sp nov., Lactobacillus songhuajiangensis sp nov and Lactobacillus nenjiangensis sp nov., isolated from Chinese traditional pickle and sourdough [J].
Gu, Chun Tao ;
Li, Chun Yan ;
Yang, Li Jie ;
Huo, Gui Cheng .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013, 63 :4698-4706
[6]   Lactobacillus heilongjiangensis sp nov., isolated from Chinese pickle [J].
Gu, Chun Tao ;
Li, Chun Yan ;
Yang, Li Jie ;
Huo, Gui Cheng .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2013, 63 :4094-4099
[7]   Lactobacillus xiangfangensis sp nov., isolated from Chinese pickle [J].
Gu, Chun Tao ;
Wang, Fang ;
Li, Chun Yan ;
Liu, Fei ;
Huo, Gui Cheng .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2012, 62 :860-863
[8]   A RAPID ANALYSIS FOR CHEMICAL GROUPING OF AEROBIC ACTINOMYCETES [J].
HASEGAWA, T ;
TAKIZAWA, M ;
TANIDA, S .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1983, 29 (04) :319-322
[9]  
Kandler O, BERGEYS MANUAL SYSTE, V2, P1209
[10]   Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes [J].
Kim, Mincheol ;
Oh, Hyun-Seok ;
Park, Sang-Cheol ;
Chun, Jongsik .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014, 64 :346-351