The relationship between intramembranous particles and aquaporin molecules in the plasma membranes of normal rat skeletal muscles: a fracture-label study

被引:4
作者
Shibuya, Seiji
Wakayama, Yoshihiro
Inoue, Masahiko
Kojima, Hiroko
Oniki, Hiroaki
机构
[1] Showa Univ, Fujigaoka Hosp, Dept Neurol, Aoba Ku, Yokohama, Kanagawa 2278501, Japan
[2] Showa Univ, Fujigaoka Hosp, Electron Microscopy Lab, Aoba Ku, Yokohama, Kanagawa 2278501, Japan
来源
JOURNAL OF ELECTRON MICROSCOPY | 2006年 / 55卷 / 02期
关键词
aquaporin; 3; 4; intramembranous particle; orthogonal array; skeletal muscle; fracture-label;
D O I
10.1093/jmicro/dfl017
中图分类号
TH742 [显微镜];
学科分类号
摘要
The plasma membrane of skeletal muscles contains water channels such as aquaporin 4 (AQP4), aquaporin 3 (AQP3) and aquaporin 7 (AQP7). In dehydrated mice, we have recently reported the altered distribution of the aggregations of intramembranous particles (IMPs), such as orthogonal array (a crystal-like structure) and IMP cluster (a rosette-like structure) on the freeze-fractured skeletal muscle plasma membranes. in this fracture-label study, we first tested whether the orthogonal arrays (OAS) were composed of AQP4 in skeletal muscles and further analyzed the relationship between IMPs including IMP clusters and AQP3 molecules. As a result, many of the gold particles indicating AQP4 was associated with OAS (79%) by our fracture-label technique. On the other hand, similar to 50% of gold particles indicating AQP3 were associated with IMP clusters. Thus we confirmed that the OAS are composed of AQP4 in skeletal muscles, and further demonstrated that some of the IMP clusters are composed of AQP3 and may participate in maintaining osmotic homeostasis in skeletal myofibers. The fracture-label method is useful in investigating the molecular identification of membrane proteins such as AQP3 and AQP4.
引用
收藏
页码:63 / 68
页数:6
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