Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography

被引：172
作者：
Dalby, MJ
Riehle, MO
Sutherland, DS
Agheli, H
Curtis, ASG
机构：
[1] Univ Glasgow, Ctr Cell Engn Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
[2] Chalmers Univ Technol, Dept Appl Phys, S-41296 Gothenburg, Sweden
来源：
BIOMATERIALS | 2004年 / 25卷 / 23期
基金：
英国生物技术与生命科学研究理事会;
关键词：
filopodia; sensing; nano-environment; fibroblasts; biomaterials;
D O I：
10.1016/j.biomaterials.2003.12.049
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In designing new biomaterials, specific chemical and topographical cues will be important in guiding cell response. Filopodia are actin-driven structures produced by cells and speculated to be involved in cell sensing of the three-dimensional environment. This report quantifies filopodia response to cylindrical nano-columns (100 nm diameter, 160 nm high) produced by colloidal lithography. Also observed were actin cytoskeleton morphology by fluorescence microscopy and filopodia morphology by electron microscopy (scanning and transmission). The results showed that the fibroblasts used produced more filopodia, per pm of cell perimeter and that filopodia could often be seen to interact with the cells' nano-environment. By understanding as to which features evoke spatial reactions in cells, it may be possible to design better biomaterials. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5415 / 5422
页数：8
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