Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line

In this study, the anti-inflammatory potential of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1 beta A (IL-1 beta), tumor necrosis factor (TNF)-alpha A, nitric oxide (NO) and IL -6. Both A. cordifolia and P. crocatum at concentration of 50 mu g/mL in cell line resulted significant decrease in TNF-alpha level (250.3 and 242.5 pg/mL respectively). A. cordifolia showed significant decrease in IL-1 beta level at 50 mu g/mL and IL-6 level at 10 mu g/mL, whilst P. crocatum showed significant decrease IL-1 beta level in three concentrations with lowest level at 50 mu g/mL. A. cordifolia showed lowest decrease in NO level at 50 mu g/mL but not comparable with normal cells, whilst P. crocatum showed significant decrease in NO level at 50 mu g/mL. This research revealed that A. cordifolia and P. crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-alpha, IL-1 beta, IL-6, and NO.