Aptamers;
Affinity separation;
Binding-induced DNA assembly;
Catalytic amplification;
Homogeneous binding assays;
Thrombin;
AFFINITY CAPILLARY-ELECTROPHORESIS;
INDUCED FLUORESCENCE POLARIZATION;
QUADRUPLEX-BASED DNAZYME;
DNA STRAND DISPLACEMENT;
HUMAN ALPHA-THROMBIN;
MAGNETIC BEADS;
ELECTROCHEMICAL DETECTION;
ULTRASENSITIVE DETECTION;
SIGNAL AMPLIFICATION;
SENSING PLATFORM;
D O I:
10.1016/j.aca.2014.04.055
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Experimentally selected single-stranded DNA and RNA aptamers are able to bind to specific target molecules with high affinity and specificity. Many analytical methods make use of affinity binding between the specific targets and their aptamers. In the development of these methods, thrombin is the most frequently used target molecule to demonstrate the proof-of-principle. This paper critically reviews more than one hundred assays that are based on aptamer binding to thrombin. This review focuses on homogeneous binding assays, electrochemical aptasensors, and affinity separation techniques. The emphasis of this review is placed on understanding the principles and unique features of the assays. The principles of most assays for thrombin are applicable to the determination of other molecular targets. (C) 2014 The Authors. Published by Elsevier B. V.
机构:
Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USAUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Chen, Yulin
Nakamoto, Kohei
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan
Univ Tsukuba, Inst Mat Sci, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, JapanUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Nakamoto, Kohei
Niwa, Osamu
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan
Univ Tsukuba, Inst Mat Sci, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, JapanUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Niwa, Osamu
Corn, Robert M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USAUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
机构:
Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USAUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Chen, Yulin
Nakamoto, Kohei
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan
Univ Tsukuba, Inst Mat Sci, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, JapanUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Nakamoto, Kohei
Niwa, Osamu
论文数: 0引用数: 0
h-index: 0
机构:
Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan
Univ Tsukuba, Inst Mat Sci, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki 3058573, JapanUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA
Niwa, Osamu
Corn, Robert M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USAUniv Calif Irvine, Dept Chem, Irvine, CA 92697 USA