Aptamer binding assays for proteins: The thrombin example-A review

被引：312

作者：

Deng, Bin ^{[1
]}

Lin, Yanwen ^{[2
]}

Wang, Chuan ^{[1
]}

Li, Feng ^{[1
]}

Wang, Zhixin ^{[1
]}

Zhang, Hongquan ^{[1
]}

Li, Xing-Fang ^{[1
]}

Le, X. Chris ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2G3, Canada

[2] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

ANALYTICA CHIMICA ACTA | 2014年 / 837卷

基金：

加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Aptamers; Affinity separation; Binding-induced DNA assembly; Catalytic amplification; Homogeneous binding assays; Thrombin; AFFINITY CAPILLARY-ELECTROPHORESIS; INDUCED FLUORESCENCE POLARIZATION; QUADRUPLEX-BASED DNAZYME; DNA STRAND DISPLACEMENT; HUMAN ALPHA-THROMBIN; MAGNETIC BEADS; ELECTROCHEMICAL DETECTION; ULTRASENSITIVE DETECTION; SIGNAL AMPLIFICATION; SENSING PLATFORM;

D O I：

10.1016/j.aca.2014.04.055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Experimentally selected single-stranded DNA and RNA aptamers are able to bind to specific target molecules with high affinity and specificity. Many analytical methods make use of affinity binding between the specific targets and their aptamers. In the development of these methods, thrombin is the most frequently used target molecule to demonstrate the proof-of-principle. This paper critically reviews more than one hundred assays that are based on aptamer binding to thrombin. This review focuses on homogeneous binding assays, electrochemical aptasensors, and affinity separation techniques. The emphasis of this review is placed on understanding the principles and unique features of the assays. The principles of most assays for thrombin are applicable to the determination of other molecular targets. (C) 2014 The Authors. Published by Elsevier B. V.

引用

页码：1 / 15

页数：15

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