Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan

被引：48

作者：

Nakano, Yuki ^{[1
]}

Kurano, Makoto ^{[1
,2
]}

Morita, Yoshifumi ^{[1
]}

Shimura, Takuya ^{[1
]}

Yokoyama, Rin ^{[1
]}

Qian, Chungen ^{[3
]}

Xia, Fuzhen ^{[4
]}

He, Fan ^{[4
]}

Kishi, Yoshiro ^{[5
]}

Okada, Jun ^{[5
]}

Yoshikawa, Naoyuki ^{[1
]}

Nagura, Yutaka ^{[6
]}

Okazaki, Hitoshi ^{[6
]}

Moriya, Kyoji ^{[7
]}

Seto, Yasuyuki ^{[8
]}

Kodama, Tatsuhiko ^{[9
]}

Yatomi, Yutaka ^{[1
,2
]}

机构：

[1] Univ Tokyo Hosp, Dept Clin Lab, Tokyo, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Clin Lab Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan

[3] Huazhong Univ Sci & Technol, Dept Biomed Engn,Key Lab Biomed Photon MOE, Coll Life Sci & Technol,Wuhan Natl Lab Optoelect, Hubei Bioinformat & Mol Imaging Key Lab,Syst Biol, Wuhan, Hubei, Peoples R China

[4] Shenzhen YHLO Biotech Co Ltd, Reagent R&D Ctr, Shenzhen, Guangdong, Peoples R China

[5] Med & Biol Labs Co Ltd, Sales & Mkt Div, Business Planning Dept, Tokyo, Japan

[6] Univ Tokyo Hosp, Dept Blood Transfus, Tokyo, Japan

[7] Univ Tokyo, Dept Infect Control & Prevent, Tokyo, Japan

[8] Univ Tokyo, Dept Gastrointestinal Surg, Tokyo, Japan

[9] Univ Tokyo, Lab Syst Biol & Med, Tokyo, Japan

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

D O I：

10.1038/s41598-021-82428-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM and IgG titers and to evaluate the sensitivity and specificity of such tests according to the specific day after the onset of COVID-19 among a patient population in Japan. We measured the titers of SARS-CoV-2 IgM and IgG in sera from 105 subjects, including 26 symptomatic COVID-19 patients, using chemiluminescent immunoassay (CLIA) methods utilizing magnetic beads coated with SARS-CoV-2 nucleocapsid protein and spike protein. The results of a ROC analysis suggested the possibility that the cutoff values in Japan might be lower than the manufacturer's reported cutoff (10 AU/mL): 1 AU/mL for IgM and 5 AU/mL for IgG. The sensitivity of the test before Day 8 after symptom onset was less than 50%; at Days 9-10, however, we obtained a much higher sensitivity of 81.8% for both IgM and IgG. At 15 days or later after symptom onset, the SARS-CoV-2 IgG test had a sensitivity of 100%. These results suggest that if the number of days since disease onset is taken into consideration, these antibody tests could be very useful for the diagnosis of COVID-19 and similar diseases.

引用

页数：10

共 23 条

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