Insulin Resistance Induced by Glucosamine in Fructose-fed Rats

被引：9

作者：

Chien, C-S. ^{[2
,3
]}

Cheng, S-C. ^{[2
]}

Wul, H-T. ^{[1
]}

Tsao, C-W. ^{[4
]}

Cheng, J-T. ^{[1
,5
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan 70101, Taiwan

[2] Chi Mei Fdn Hosp, Dept Orthopaed, Tainan, Taiwan

[3] Natl Cheng Kung Univ, Coll Med, Inst Biomed Engn, Tainan 70101, Taiwan

[4] Chung Hwa Univ Med Technol, Dept Nursing, Tainan, Taiwan

[5] Chi Mei Fdn Hosp, Dept Med Res, Tainan, Taiwan

来源：

HORMONE AND METABOLIC RESEARCH | 2009年 / 41卷 / 07期

关键词：

FXR; glucosamine; glucose transporter GLUT4; insulin resistance; glucose tolerance test; FARNESOID-X-RECEPTOR; SKELETAL-MUSCLE; ORAL GLUCOSAMINE; IN-VIVO; DIFFERENT MECHANISMS; GLYCOGEN-SYNTHASE; ACTIVATION; INFUSION; GLYCOSYLATION; METABOLISM;

D O I：

10.1055/s-0029-1216344

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Glucosamine has been widely used to treat osteoporosis in clinic and it shows an effect on hexosamine biosynthetic pathway in glucose-induced insulin resistance. However, glucosamine and chronic hyperglycemia is not correlative. Thus, we used C2C12 cell line to carry out the uptake assay of 2-[C-14]-deoxy-d-glucose and [H-3]-glucosamine. Glucosamine inhibited the in vitro glucose uptake in a concentration-dependent manner. The glucose transporter GLUT4 prepared for [H-3]-glucosamine uptake showed a concentration-dependent competition between glucose and glucosamine uptake. The effects of glucosamine on glucose tolerance and homeostasis model assessment (HOMA) were also determined in normal Wistar and fructose-fed rats. Both plasma glucose levels and/or HOMA index were not changed in normal rats treated with glucosamine as compared with the saline-treated control. However, we found that glucosamine exhibited an effect on the expression of farnesoid X receptor in liver to exacerbate the values of HOMA and accelerate the development of insulin resistance in fructose-fed rats. Thus, glucosamine should be applied with caution in type-2 diabetic patients.

引用

页码：542 / 547

页数：6

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