Chronic ethanol ingestion increases efficiency of oxidative phosphorylation in rat liver mitochondria

被引：20
作者：
Piquet, MA
Nogueira, V
Devin, A
Sibille, B
Filippi, C
Fontaine, E
Roulet, M
Rigoulet, M
Leverve, XM
机构：
[1] UJF, Lab Bioenerget Fondamental & Appl, F-38041 Grenoble, France
[2] CHU Vaudois, Lab Nutr Clin, CH-1011 Lausanne, Switzerland
[3] CNRS, Inst Biochim & Genet Cellulaires, F-33077 Bordeaux, France
来源：
FEBS LETTERS | 2000年 / 468卷 / 2-3期
关键词：
mitochondrion; oxidative phosphorylation; ethanol; cytochrome oxidase; proton-motive force; sodium azide;
D O I：
10.1016/S0014-5793(00)01225-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding protonmotive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative-phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol, Cytochrome oxidase inhibition by sodium-azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation. (C) 2000 Federation of European Biochemical Societies.
引用
收藏
页码：239 / 242
页数：4
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