A chemotaxonomic study of the lipoglycans of Rhodococcus rhodnii N445 (NCIMB 11279)

Rhodococcus rhodnii N445 was investigated for the presence of macroamphiphilic lipoglycan. Purification of a hot phenol-water extract by hydrophobic interaction chromatography allowed the resolution of three lipoglycan fractions. The two main preparations contained lipoglycans with carbohydrate compositions consistent with the presence of lipoarabinomannan and lipomannan, whilst the minor fraction appeared to contain a mixture of these two lipoglycans. The fatty acid composition of the lipoglycans resembled that of the whole cells except that the relative proportion of unsaturated fatty acids was decreased. Although lipoarabinomannan and structurally-related lipomannan lipoglycans from representatives of the genus Mycobacterium have been extensively studied, this is the first report of the lipoglycan composition of a representative of the genus Rhodococcus as presently defined. These findings provide further chemotaxonomic evidence that lipoarabinomannan-type lipoglycans are widely distributed throughout the mycolic acid-containing actinomycetes.