Facile synthesis of PSMA-g-3ABA/MWCNTs nanocomposite as a substrate for hemoglobin immobilization: Application to catalysis of H2O2

被引：51

作者：

Baghayeri, Mehdi ^{[1
]}

Zare, Ehsan Nazarzadeh ^{[2
]}

Hasanzadeh, Reza ^{[3
]}

机构：

[1] Hakim Sabzevari Univ, Dept Chem, Fac Sci, Sabzevar, Iran

[2] Univ Mazandaran, Dept Polymer Chem, Fac Chem, Babol Sar, Iran

[3] Urmia Univ, Dept Chem, Fac Sci, Orumiyeh, Iran

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2014年 / 39卷

关键词：

Poly(styrene-alternative-maleic anhydride); Hemoglobin; Nanocomposite; Biosensor; Electrocatalysis; TEMPERATURE IONIC LIQUID; DIRECT ELECTRON-TRANSFER; MULTIWALLED CARBON NANOTUBES; DIRECT ELECTROCHEMISTRY; AMPEROMETRIC BIOSENSOR; VOLTAMMETRIC DETERMINATION; GLUCOSE-OXIDASE; COMPOSITE FILM; 2,7-BIS(FERROCENYL ETHYL)FLUOREN-9-ONE; METAL-IONS;

D O I：

10.1016/j.msec.2014.03.012

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

The new nanocomposite films based on poly(styrene-alternative-maleic anhydride) grafted to 3-aminobenzoic acid (PSMA-g-3ABA) and multi-walled carbon nanotubes (MWCNTs) were applied to immobilize hemoglobin (Hb) for biosensor fabrication (PSMA-g-3ABA/MWCNTs). Electrochemical impedance spectroscopy was used to confirm the adsorption of Hb onto the surface of PSMA-g-3ABA/MWCNTs. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The biosensor was used to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H2O2 concentration ranging widely from 1.0 x 10(-6) M to 5.0 x 10(-4) M with a detection limit of 3.2 x 10(-7) M. The apparent Michaelis-Menten constant of Hb on the modified electrode was estimated to be 0.22 mM. The proposed method opens a way to develop biosensors by using nanostructured materials with low electrical conductivity. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：213 / 220

页数：8

共 67 条

[1] Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme
Ahmad, A
Akhtar, MS
Bhakuni, V
[J]. BIOCHEMISTRY, 2001, 40 (07) : 1945 - 1955
[2] Materials science - Nanotube composites
Ajayan, Pulickel M.
Tour, James M.
[J]. NATURE, 2007, 447 (7148) : 1066 - 1068
[3] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS
ARMSTRONG, FA
HILL, HAO
WALTON, NJ
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1988, 21 (11) : 407 - 413
[4] A simple hydrogen peroxide biosensor based on a novel electro-magnetic poly(p-phenylenediamine)@Fe3O4 nanocomposite
Baghayeri, Mehdi
Zare, Ehsan Nazarzadeh
Lakouraj, Moslem Mansour
[J]. BIOSENSORS & BIOELECTRONICS, 2014, 55 : 259 - 265
[5] Direct electrochemistry and electrocatalysis of hemoglobin immobilized on biocompatible poly(styrene-alternative-maleic acid)/functionalized multi-wall carbon nanotubes blends
Baghayeri, Mehdi
Zare, Ehsan Nazarzadeh
Namadchian, Melika
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2013, 188 : 227 - 234
[6] Application of a Carbon-Paste Electrode Modified with 2,7-Bis(ferrocenyl ethyl)fluoren-9-one and Carbon Nanotubes for Voltammetric Determination of Levodopa in the Presence of Uric Acid and Folic Acid
Beitollahi, Hadi
Raoof, Jahan-Bakhsh
Hosseinzadeh, Rahman
[J]. ELECTROANALYSIS, 2011, 23 (08) : 1934 - 1940
[7] Electrochemical behavior and voltammetric determination of pyrazinamide using a poly-histidine modified electrode
Bergamini, Marcio F.
Santos, Daniela P.
Zanoni, Maria Valnice B.
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2013, 690 : 47 - 52
[8] BOND AM, 1980, MODERN POLAROGRAPHIC, P27
[9] Recent Advances in Research on Carbon Nanotube-Polymer Composites
Byrne, Michele T.
Gun'ko, Yurii K.
[J]. ADVANCED MATERIALS, 2010, 22 (15) : 1672 - 1688
[10] Direct electron transfer and bioelectrocatalysis of hemoglobin at a carbon nanotube electrode
Cai, CX
Chen, J
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 325 (02) : 285 - 292

← 1 2 3 4 5 6 7 →