Preparation of A Novel Zwitterionic Hybrid Monolith Column with Incorporated Fumed Silica Nanoparticle and Its Application in Pressurized Capillary Electrochromatography

The hybrid poly (GMA-EDMA-MFSNP) monolithic column was prepared by in-situ copolymerization of gamma-(methylacryloyloxy) propyltrimethoxysilane modified fumed silica nanoparticles with glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EDMA) as monomers, cyclohexanol and dodecanol as binary porogenic agent, and azobisisobutyronitrile (AIBN) as initiator. With the ring-opening reaction of glycidyl methacrylate (GMA), phenylalanine was chemically bonded on hybrid monolithic column. To obtain high efficiency, permeability and electroosmotic flow (EOF), the contents of the polymerization mixture and the chemical modification conditions of phenylalanine were optimized. The column prepared under the optimal conditions was a zwitterionic monolith with a mix-mode mechanism of reversed-phase and anion-exchange. By using the column prepared here, various analytes such as alkybenzenes, polycyclic aromatic hydrocarbons, benzoic acid analytes, phenol analytes and peptides were successfully separated in pressurized capillary electrochromatography. The column efficiency for toluene was up to 122592.