Characterization and antioxidant activities of degraded polysaccharides from Poria cocos sclerotium

被引：82
作者：
Tang, Jin [1 ]
Nie, Jing [2 ]
Li, Danping [2 ]
Zhu, Wenjun [1 ]
Zhang, Shaopeng [1 ]
Ma, Fang [1 ]
Sun, Qiao [1 ]
Song, Jia [1 ]
Zheng, Yonglian [1 ]
Chen, Ping [1 ]
机构：
[1] Wuhan Polytech Univ, Sch Biol & Pharm Engn, Wuhan 430023, Peoples R China
[2] Hubei Inst Food & Drug Control, Wuhan 430023, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2014年 / 105卷
基金：
中国国家自然科学基金;
关键词：
Poria cocos FAWolf; Degradation; Polysaccharide; Antioxidant activity; CARBOXYMETHYLATED DERIVATIVES; ANTITUMOR ACTIVITIES; LENTINUS-EDODES; URONIC-ACID; EXTRACTION; SUGARS;
D O I：
10.1016/j.carbpol.2014.01.049
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Poria cocos F.A.Wolf is a Chinese traditional medicine used to treat chronic gastritis, edema, nephrosis, gastric atony, and acute gastroenteric catarrh. Polysaccharides are the main active component of P. cocos. We obtained polysaccharides PCP-1, PCP-2, and PCP-3 from the degradation of P. cocos polysaccharides (PCP) with different concentrations of H2O2 solution. Molecular weights were determined by high performance size exclusion chromatography. HPLC analysis of monosaccharide composition confirmed that PCP-I, PCP-2, and PCP-3 are heteropolysaccharides composed of glucose and arabinose. IR spectra indicated obvious characteristic peaks of polysaccharides. The antioxidant activities of these polysaccharides were evaluated by established in vitro systems, including scavenging activity of hydroxyl radicals, ABTS radicals, and ferrous ions. The degradation polysaccharides exhibited obvious and concentration-dependent antioxidant properties. In addition, DNA binding analysis showed that PCP-1 had a stronger capacity than other polysaccharides to interact with DNA. However, each polysaccharide had a certain capacity for DNA damage protection. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：121 / 126
页数：6
相关论文
共 42 条
  • [1] Assessment of DNA damage induced by extracts, fractions and isolated compounds of Davilla nitida and Davilla elliptica (Dilleniaceae)
    Biso, Fabiana Izilda
    Rodrigues, Clenilson Martins
    Rinaldo, Daniel
    dos Reis, Mariana Bisarro
    Bernardi, Caroline Cristiane
    Pelielo de Mattos, Jose Carlos
    Caldeira-de-Araujo, Adriano
    Vilegas, Wagner
    de Syllos Colus, Ilce Mara
    Varanda, Eliana Aparecida
    [J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2010, 702 (01) : 92 - 99
  • [2] A MODIFIED URONIC ACID CARBAZOLE REACTION
    BITTER, T
    MUIR, HM
    [J]. ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) : 330 - &
  • [3] SPECTROPHOTOMETRIC DETERMINATION OF SERUM IRON AT SUBMICROGRAM LEVEL WITH A NEW REAGENT (FERROZINE)
    CARTER, P
    [J]. ANALYTICAL BIOCHEMISTRY, 1971, 40 (02) : 450 - &
  • [4] Antioxidant activities of polysaccharides from Lentinus edodes and their significance for disease prevention
    Chen, Huoliang
    Ju, Ying
    Li, Junjie
    Yu, Min
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 50 (01) : 214 - 218
  • [5] Chain conformation and anti-tumor activities of phosphorylated (1→3)-β-D-glucan from Poria cocos
    Chen, Xiaoyu
    Xu, Xiaojuan
    Zhang, Lina
    Zeng, Fanbo
    [J]. CARBOHYDRATE POLYMERS, 2009, 78 (03) : 581 - 587
  • [6] CHIHARA G, 1970, CANCER RES, V30, P2776
  • [7] Multivariate analysis of uronic acid and neutral sugars in whole pectic samples by FT-IR spectroscopy
    Coimbra, MA
    Barros, A
    Barros, M
    Rutledge, DN
    Delgadillo, I
    [J]. CARBOHYDRATE POLYMERS, 1998, 37 (03) : 241 - 248
  • [8] Ding Q, 1998, CHINESE J POLYM SCI, V16, P62
  • [9] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS
    DUBOIS, M
    GILLES, K
    HAMILTON, JK
    REBERS, PA
    SMITH, F
    [J]. NATURE, 1951, 168 (4265) : 167 - 167
  • [10] GORNALL AG, 1949, J BIOL CHEM, V177, P751
12345