Superior Fluorescent Probe for Detection of Cardiolipin

被引:51
|
作者
Leung, Chris W. T. [1 ,2 ]
Hong, Yuning [1 ,2 ,3 ]
Hanske, Jonas [3 ,4 ]
Zhao, Engui [1 ,2 ]
Chen, Sijie [1 ,2 ]
Pletneva, Ekaterina V. [3 ]
Tang, Ben Zhong [1 ,2 ,5 ]
机构
[1] Hong Kong Univ Sci & Technol, Div Life Sci, State Key Lab Mol Neurosci, Inst Adv Study,Div Biomed Engn,Dept Chem, Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Inst Mol Funct Mat, Kowloon, Hong Kong, Peoples R China
[3] Dartmouth Coll, Dept Chem, Hanover, NH 03755 USA
[4] Free Univ Berlin, Dept Biol Chem & Pharm, Inst Chem & Biochem, D-14195 Berlin, Germany
[5] S China Univ Technol, Guangdong Innovat Res Team, SCUT HKUST Joint Res Lab, State Key Lab Luminescent Mat & Devices, Guangzhou 510640, Guangdong, Peoples R China
基金
美国国家卫生研究院;
关键词
AGGREGATION-INDUCED EMISSION; CYTOCHROME-C; SELECTIVITY;
D O I
10.1021/ac403616c
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cardiolipin (CL) is a unique phospholipid found in mitochondrial inner membrane. It is a key component for mitochondrial function in both respiration and apoptosis. The level of CL is an important parameter for investigating these intracellular events and is a critical indicator of a number of diseases associated with mitochondrial respiratory functions. 10-Nonyl acridine orange (NAO) is the only fluorescent dye currently available for CL detection. However, the performance of NAO is far from satisfactory in terms of selectivity and sensitivity. In this work, we report an aggregation-induced emission-active fluorogen, TTAPE-Me, for CL detection and quantification. With improved sensitivity and excellent selectivity to CL over other major mitochondria] membrane lipids, TTAPE-Me could serve as a valuable fluorescent sensor for CL quantification. The use of TTAPE-Me for the quantification of isolated mitochondria is also demonstrated.
引用
收藏
页码:1263 / 1268
页数:6
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