Transcriptome analysis of the salivary glands of the female tick Amblyomma americanum (Acari: Ixodidae)

被引:44
作者
Aljamali, M. N. [1 ]
Hern, L. [3 ]
Kupfer, D. [3 ]
Downard, S. [3 ]
So, S. [3 ]
Roe, B. A. [3 ]
Sauer, J. R. [2 ]
Essenberg, R. C. [1 ]
机构
[1] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
[2] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA
[3] Univ Oklahoma, Dept Chem, Norman, OK 73019 USA
关键词
female tick; salivary glands; Ambyomma americanum; expressed sequence tags; LONE STAR TICK; PLATELET-AGGREGATION INHIBITORS; EXPRESSED SEQUENCE TAGS; IXODES-SCAPULARIS; CATTLE TICK; MOLECULAR-CLONING; PROTEIN; GENE; SIALOME; EXTRACT;
D O I
10.1111/j.1365-2583.2009.00863.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ticks infest a wide range of hosts while bypassing their immune, inflammatory and haemostatic responses during their extended feeding, which may last for more than two weeks. Here, we present a transcriptome analysis of 3868 expressed sequence tags (ESTs) from three cDNA libraries generated from the salivary glands of adult female Ambyomma americanum ticks at different stages of feeding. We applied a normalization step for one library, significantly decreasing the abundance of mitochondrial sequences amongst the 2292 sequences from the normalized library. Our ESTs include homologues that may modulate haemostatic, immune and inflammatory responses of the hosts. Other ESTs probably represent important components of the highly efficient secretory pathways for salivary proteins and concomitantly transmitted pathogens.
引用
收藏
页码:129 / 154
页数:26
相关论文
共 73 条
  • [1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT
    ADAMS, MD
    KELLEY, JM
    GOCAYNE, JD
    DUBNICK, M
    POLYMEROPOULOS, MH
    XIAO, H
    MERRIL, CR
    WU, A
    OLDE, B
    MORENO, RF
    KERLAVAGE, AR
    MCCOMBIE, WR
    VENTER, JC
    [J]. SCIENCE, 1991, 252 (5013) : 1651 - 1656
  • [2] α1-Microglobulin:: a yellow-brown lipocalin
    Åkerström, B
    Lögdberg, L
    Berggård, T
    Osmark, P
    Lindqvist, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2000, 1482 (1-2): : 172 - 184
  • [3] Transcriptome analysis of the salivary glands of Dermacentor andersoni Stiles (Acari: Ixodidae)
    Alarcon-Chaidez, Francisco J.
    Sun, Jianxin
    Wikel, Stephen K.
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 37 (01) : 48 - 71
  • [4] Identity and synthesis of prostaglandins in the lone star tick, Amblyomma americanum (L.), as assessed by radio-immunoassay and gas chromatography/mass spectrometry
    Aljamali, M
    Bowman, AS
    Dillwith, JW
    Tucker, JS
    Yates, GW
    Essenberg, RC
    Sauer, JR
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2002, 32 (03) : 331 - 341
  • [5] RNA interference in ticks:: a study using histamine binding protein dsRNA in the female tick Amblyomma americanum
    Aljamali, MN
    Bior, AD
    Sauer, JR
    Essenberg, RC
    [J]. INSECT MOLECULAR BIOLOGY, 2003, 12 (03) : 299 - 305
  • [6] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [7] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [8] ANTICOAGULANT IN THE TICK IXODES-HOLOCYCLUS
    ANASTOPOULOS, P
    THURN, MJ
    BROADY, KW
    [J]. AUSTRALIAN VETERINARY JOURNAL, 1991, 68 (11) : 366 - 367
  • [9] [Anonymous], 1997, CELL MOL APPROACH
  • [10] PipeOnline 2.0: automated EST processing and functional data sorting
    Ayoubi, P
    Jin, XJ
    Leite, S
    Liu, XH
    Martajaja, J
    Abduraham, A
    Wan, QL
    Yan, W
    Misawa, E
    Prade, RA
    [J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (21) : 4761 - 4769