Argonaute-Bound Small RNAs from Promoter-Proximal RNA Polymerase II

被引：88
作者：
Zamudio, Jesse R. [1 ]
Kelly, Timothy J. [1 ]
Sharp, Phillip A. [1 ,2 ]
机构：
[1] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA
[2] MIT, Dept Biol, Cambridge, MA 02139 USA
来源：
CELL | 2014年 / 156卷 / 05期
关键词：
TRANSCRIPTION; GENE; EXPRESSION; MICRORNAS; DNA; INTERFERENCE; TERMINATION; MECHANISMS; INITIATION; SIRNAS;
D O I：
10.1016/j.cell.2014.01.041
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Argonaute (Ago) proteins mediate posttranscriptional gene repression by binding guide miRNAs to regulate targeted RNAs. To confidently assess Ago-bound small RNAs, we adapted a mouse embryonic stem cell system to express a single epitope-tagged Ago protein family member in an inducible manner. Here, we report the small RNA profile of Ago-deficient cells and show that Ago-dependent stability is a common feature of mammalian miRNAs. Using this criteria and immunopurification, we identified an Ago-dependent class of noncanonical miRNAs derived from protein-coding gene promoters, which we name transcriptional start site miRNAs (TSS-miRNAs). A subset of promoter-proximal RNA polymerase II (RNAPII) complexes produces hairpin RNAs that are processed in a DiGeorge syndrome critical region gene 8 (Dgcr8)/Drosha-independent but Dicer-dependent manner. TSS-miRNA activity is detectable from endogenous levels and following overexpression of mRNA constructs. Finally, we present evidence of differential expression and conservation in humans, suggesting important roles in gene regulation.
引用
收藏
页码：920 / 934
页数：15
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