High-performance liquid chromatography determination of phenolic components in wine using off-line isotachophoretic pretreatment

被引：25
作者：
Sladkovsky, R
Solich, P
Urbánek, M
机构：
[1] Charles Univ, Fac Pharm, Dept Analyt Chem, Hradec Kralove 50005, Czech Republic
[2] Acad Sci Czech Republ, Inst Analyt Chem, Brno 61142, Czech Republic
来源：
JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1040卷 / 02期
关键词：
wine; food analysis; isotachophoresis; sample preparation; phenolic compounds; organic acids;
D O I：
10.1016/j.chroma.2004.04.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The off-line combination of isotachophoresis (ITP) and high-performance liquid chromatography (HPLC) to improve sample pretreatment and determination of phenolic compounds in wine was investigated. The ITP system provided an enhanced sample load capacity and served as a sample clean-up technique, HPLC performed a final separation of the analytes presented in samples. The phenolic components were separated by Discovery RP Amid C-16 chromatography column using water-methanol-acetonitrile-orthophosphofic acid gradient. The identification of phenolic compounds was made by comparison of the retention data obtained for the standard mixture, pretreated sample and the sample spiked standard additions. Satisfactory recoveries for all components analysed were observed between 86.1 and 109.2%. (C) 2004 Elsevier B.V. All rights reserved.
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收藏
页码：179 / 184
页数：6
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