Ligation Chain Reaction based gold nanoparticle assembly for ultrasensitive DNA detection

被引：28

作者：

Yin, Honghong ^{[1
,2
]}

Huang, Xin ^{[2
]}

Ma, Wei ^{[1
]}

Xu, Liguang ^{[1
]}

Zhu, Shuifang ^{[2
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Chinese Acad Inspect & Quarantine, Inst Plant Quarantine, Beijing 100029, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 52卷

关键词：

Ligation Chain Reaction; Gold nanoparticle; Assembly; Ultrasensitive; DNA detection; COLORIMETRIC DETECTION; OPTICAL-PROPERTIES; PCR; SEQUENCES;

D O I：

10.1016/j.bios.2013.07.064

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A simple and ultrasensitive DNA biosensor was developed utilizing the color and size changes originating from Ligation Chain Reaction (LCR)-based gold nanoparticle assembly as an indicator. Using synthetic DNA of 10-fold serial dilutions as a target, the limit of detection (LOD) was 1.5 aM with a range of DNA concentrations from 0.01 to 1000 fM by UV-vis absorption and 0.1 aM in the range of 0.01-10,000 fM by dynamic light scattering (DLS). This developed method is low-cost, rapid, and much lower LOD for the detection of specific short DNA sequences, and the results can be directly determined either by the color or by the DLS. The capability of high-throughput detection with the aid of PCR amplification apparatus can also be realized. (C) 2013 Published by Elsevier B.V.

引用

页码：8 / 12

页数：5

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