The demand for NO, after-treatment system has increased dramatically due to the stricter NO emission regulations for diesel vehicles. The urea-SCR system is one of the NO after-treatment methods found to be quite effective to meet the regulation requirement enforced by various authorities including the Euro-6. In order to develop an effective urea-SCR system, it is critical to establish an even distribution of reductant over the catalyst surface since this favorable distribution can increase reduction reaction and in turn, improve NO conversion efficiencies. In the current study, a number of design variations of the urea-SCR system which included two mixer types and three decomposition pipe lengths, were evaluated systematically using CFD analysis and experimental measurements. The purpose of the CFD analysis was to estimate the distribution of reductant within the urea-SCR system with a specific configuration and the purpose of the engine emission test was to measure the amount of NO, reduction, respectively. The results from the systematic analysis revealed the relation between the reductant distribution over the SCR and the performance of the NO reduction.