Theoretical and experimental comparison of mobile phase consumption between ultra-high-performance liquid chromatography and high performance liquid chromatography

被引：36

作者：

Chen, Shujun ^{[1
]}

Kord, Alireza ^{[1
]}

机构：

[1] GlaxoSmithKline, Chem Dev, Analyt Sci, King Of Prussia, PA 19406 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 34期

关键词：

Ultra performance liquid chromatography; Small particles; Very high pressure; Mobile phase consumption; SOLUTE RETENTION; PRESSURE; UPLC;

D O I：

10.1016/j.chroma.2009.06.084

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ultra performance liquid chromatography (UPLC) using small particles and very high pressure has demonstrated higher resolution and speed compared with conventional HPLC. An additional benefit of UPLC is the significantly reduced consumption of mobile phase. This report discusses how column length, particle size, inner column diameter, extra column void Volume, and capacity factor contribute to the reduction of mobile phase consumption in UPLC compared with HPLC. In addition, theoretical and experimental comparison of mobile phase Consumption was made between isocratic HPLC and UPLC as well as between gradient HPLC and UPLC. Both theoretical and experimental results indicate that UPLC typically saves at least 80% of mobile phase in isocratic and gradient conditions when compared with HPLC. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：6204 / 6209

页数：6

共 17 条

[1] New and rapid Ultra-Performance Liquid Chromatography assay of paclitaxel [J].

Alvi, Khisal A. ;

Wang, Songjie ;

Tous, Guillermo .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2008, 31 (07) :941-949

[2] Influence of frictional heating on temperature gradients in ultra-high-pressure liquid chromatography on 2.1 mm ID columns [J].

de Villiers, A ;

Lauer, H ;

Szucs, R ;

Goodall, S ;

Sandra, P .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1113 (1-2) :84-91

[3] Validated UPLC method for the fast and sensitive determination of steroid residues in support of cleaning validation in formulation area [J].

Fekete, Szabolcs ;

Fekete, Jeno ;

Ganzler, Katalin .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2009, 49 (03) :833-838

[4] INFLUENCE OF PRESSURE ON SOLUTE RETENTION IN LIQUID-CHROMATOGRAPHY [J].

GUIOCHON, G ;

SEPANIAK, MJ .

JOURNAL OF CHROMATOGRAPHY, 1992, 606 (02) :248-250

[5]

Lippert JA, 1999, J MICROCOLUMN SEP, V11, P631, DOI 10.1002/(SICI)1520-667X(199911)11:9<631::AID-MCS1>3.0.CO

[6]

2-I

[7] THEORY OF SOLUTE RETENTION IN LIQUID-LIQUID CHROMATOGRAPHY [J].

LOCKE, DC ;

MARTIRE, DE .

ANALYTICAL CHEMISTRY, 1967, 39 (08) :921-&

[8] Ultrahigh pressure reversed-phase capillary liquid chromatography:: Isocratic and gradient elution using columns packed with 1.0-μm particles [J].

MacNair, JE ;

Patel, KD ;

Jorgenson, JW .

ANALYTICAL CHEMISTRY, 1999, 71 (03) :700-708

[9] Ultrahigh pressure reversed-phase liquid chromatography in packed capillary columns [J].

MacNair, JE ;

Lewis, KC ;

Jorgenson, JW .

ANALYTICAL CHEMISTRY, 1997, 69 (06) :983-989

[10]

Neue U.D., 1997, HPLC Columns: Theory, Technology, and Practice

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