The role of C/EBPδ in the early stages of adipogenesis

被引:80
作者
Hishida, Tomoaki [1 ]
Nishizuka, Makoto [1 ]
Osada, Shigehiro [1 ]
Imagawa, Masayoshi [1 ]
机构
[1] Nagoya City Univ, Dept Mol Biol, Grad Sch Pharmaceut Sci, Mizuho Ku, Aichi 4678603, Japan
基金
日本学术振兴会;
关键词
Adipocyte differentiation; Obesity; Peroxisome proliferator-activated receptor gamma; CCAAT/enhancer-binding protein; 3T3-L1; cell; MITOTIC CLONAL EXPANSION; BINDING PROTEIN-BETA; ADIPOCYTE DIFFERENTIATION; 3T3-L1; CELLS; ADIPOSE CONVERSION; CRUCIAL ROLE; GENE; ACTIVATION; EXPRESSION; PLAYS;
D O I
10.1016/j.biochi.2009.02.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adipocyte differentiation is a complex process triggered and facilitated by transcription factors such as peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer-binding protein (C/EBP) alpha. Most about the cascade underlying the differentiation process, especially events in the early stages, remain to be elucidated. Early on in adipocyte differentiation, the C/EBP beta and C/EBP delta genes are rapidly induced to express and later activate PPAR gamma and C/EBP alpha expression. C/EBP beta also plays a crucial role in mitotic clonal expansion (MCE), the approximately two rounds of mitosis which occurs soon after pre-adipocytes are stimulated by differentiation inducers and a necessary step for adipocyte differentiation. However, the effect of C/EBP delta, another member of the C/EBP family, on MCE remains unclear. In the present study, we investigated the role of C/EBP delta in the early stages of adipogenesis. A remarkable induction of C/EBP delta gene expression after the initiation of differentiation was observed not in proliferating preadipocytes, but in growth-arrested, differentiable cells. RNAi-mediated knockdown of C/EBP delta dramatically suppressed cell growth after differentiation was induced, and inhibited conversion into lipid-laden adipocytes. Furthermore, silencing of C/EBP delta impaired the expression of factor for adipocyte differentiation (fad) 49, which is up-regulated and plays a crucial role early in adipogenesis. Taken together, these findings show that C/EBP delta is involved in MCE and gene expression in the early stages of adipocyte differentiation. (C) 2009 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:654 / 657
页数:4
相关论文
共 17 条
  • [1] REGULATED EXPRESSION OF 3 C/EBP ISOFORMS DURING ADIPOSE CONVERSION OF 3T3-L1 CELLS
    CAO, ZD
    UMEK, RM
    MCKNIGHT, SL
    [J]. GENES & DEVELOPMENT, 1991, 5 (09) : 1538 - 1552
  • [2] The role of C/EBP genes in adipocyte differentiation
    Darlington, GJ
    Ross, SE
    MacDougald, OA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (46) : 30057 - 30060
  • [3] ESTABLISHED PRE-ADIPOSE CELL LINE AND ITS DIFFERENTIATION IN CULTURE .2. FACTORS AFFECTING ADIPOSE CONVERSION
    GREEN, H
    KEHINDE, O
    [J]. CELL, 1975, 5 (01) : 19 - 27
  • [4] A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion
    Hishida, Tomoaki
    Eguchi, Tsuyoshi
    Osada, Shigehiro
    Nishizuka, Makoto
    Imagawa, Masayoshi
    [J]. FEBS JOURNAL, 2008, 275 (22) : 5576 - 5588
  • [5] peg10, an imprinted gene, plays a crucial role in adipocyte differentiation
    Hishida, Tomoaki
    Naito, Kumiko
    Osada, Shigehiro
    Nishizuka, Makoto
    Imagawa, Masayoshi
    [J]. FEBS LETTERS, 2007, 581 (22) : 4272 - 4278
  • [6] Identification of inducible genes at the early stage of adipocyte differentiation of 3T3-L1 cells
    Imagawa, M
    Tsuchiya, T
    Nishihara, T
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 254 (02) : 299 - 305
  • [7] FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication
    Johmura, Yoshikazu
    Osada, Shigehiro
    Nishizuka, Makoto
    Imagawa, Masayoshi
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (04) : 2265 - 2274
  • [8] Obesity as a medical problem
    Kopelman, PG
    [J]. NATURE, 2000, 404 (6778) : 635 - 643
  • [9] Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells
    Nishizuka, M
    Tsuchiya, T
    Nishihara, T
    Imagawa, M
    [J]. BIOCHEMICAL JOURNAL, 2002, 361 : 629 - 633
  • [10] Mitotic clonal expansion during preadipocyte differentiation: Calpain-mediated turnover of p27
    Patel, YM
    Lane, MD
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) : 17653 - 17660