The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis

被引：2
|
作者：
Churchill-Angus, Alicia M. [1 ]
Sedelnikova, Svetlana E. [1 ]
Schofield, Thomas H. B. [1 ,2 ]
Baker, Patrick J. [1 ]
机构：
[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England
[2] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2020年 / 76卷
基金：
英国工程与自然科学研究理事会;
关键词：
pore-forming toxin; crystallization; ClyA family; Serratia; RAY CRYSTAL-STRUCTURE; BACILLUS-CEREUS NHE; HEMOLYSIN-BL; COMPLEX; SHEA; CLYA;
D O I：
10.1107/S2053230X20013862
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tripartite alpha-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative alpha-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.
引用
收藏
页码：577 / 582
页数：6
相关论文
共 50 条
  • [41] PEPTIDES FROM THE N-TERMINAL DOMAIN OF A PORE-FORMING TOXIN, STICHOLYSIN II. CONFORMATION AND ACTIVITY
    Schreier, S.
    Casallanovo, F.
    de Oliveira, F. F.
    Souto, A. L. C. F.
    de Souza, F. C.
    Cilli, E. M.
    Martinez, Y.
    Martinez, J.
    Lanio, M. E.
    Alvarez, C.
    JOURNAL OF PEPTIDE SCIENCE, 2004, 10 : 279 - 279
  • [42] Localization and environment of tryptophans in soluble and membrane-bound states of a pore-forming toxin from Staphylococcus aureus
    Raja, SM
    Rawat, SS
    Chattopadhyay, A
    Lala, AK
    BIOPHYSICAL JOURNAL, 1999, 76 (03) : 1469 - 1479
  • [43] Crystal structure of the soluble form of equinatoxin II, a pore-forming toxin from the sea anemone Actinia equina
    Athanasiadis, A
    Anderluh, G
    Macek, P
    Turk, D
    STRUCTURE, 2001, 9 (04) : 341 - 346
  • [44] Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata
    Lassalle, Damien
    Tetreau, Guillaume
    Pinaud, Silvain
    Galinier, Richard
    Crickmore, Neil
    Gourbal, Benjamin
    Duval, David
    GENES, 2020, 11 (01)
  • [45] Protective role of autophagy against Vibrio cholerae cytolysin, a pore-forming toxin from V-cholerae
    Gabriel Gutierrez, Maximiliano
    Saka, Hector Alex
    Chinen, Isabel
    Zoppino, Felipe C. M.
    Yoshimori, Tamotsu
    Bocco, Jose Luis
    Colombo, Maria Isabel
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (06) : 1829 - 1834
  • [46] Structural and functional analysis of a pore forming toxin from Streptococcus mitis
    Patil, Sanket B.
    Das, Parijat
    Badgujar, Dilip C.
    Bhaumik, Prasenjit
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2024, 80
  • [47] PURIFICATION AND CHARACTERIZATION OF A CYTOLYTIC PORE-FORMING PROTEIN FROM GRANULES OF CLONED LYMPHOCYTES WITH NATURAL-KILLER ACTIVITY
    YOUNG, JDE
    HENGARTNER, H
    PODACK, ER
    COHN, ZA
    CELL, 1986, 44 (06) : 849 - 859
  • [48] Caenorhabditis elegans LIN-24, a homolog of bacterial pore-forming toxin, protects the host from microbial infection
    Zhang, Huijie
    Zeng, Weirong
    Zhao, Ming-Ming
    Wang, Jiali
    Wang, Qiquan
    Chen, Ting
    Zhang, Yuyan
    Lee, Wenhui
    Chen, Shenghan
    Zhang, Yun
    Lan, Xinqiang
    Xiang, Yang
    FASEB JOURNAL, 2023, 37 (10):
  • [49] Lysine 77 is a Key Residue in Aggregation of Equinatoxin II, a Pore-forming Toxin from Sea Anemone Actinia equina
    G. Anderluh
    A. Barlič
    C. Potrich
    P. Maček
    G. Menestrina
    The Journal of Membrane Biology, 2000, 173 : 47 - 55
  • [50] Lipid phase coexistence favors membrane insertion of equinatoxin-II, a pore-forming toxin from Actinia equina
    Barlic, A
    Gutiérrez-Aguirre, I
    Caaveiro, JMM
    Cruz, A
    Ruiz-Argüello, MB
    Pérez-Gil, J
    González-Mañas, JM
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (33) : 34209 - 34216
12345