Extraction and characterization of acid-soluble collagen from scales and skin of tilapia (Oreochromis niloticus)

被引：156

作者：

Chen, Junde ^{[1
]}

Li, Long ^{[1
]}

Yi, Ruizao ^{[1
]}

Xu, Nuohua ^{[1
]}

Gao, Ran ^{[1
]}

Hong, Bihong ^{[1
]}

机构：

[1] State Ocean Adm, Inst Oceanog 3, Marine Biol Resource Comprehens Utilizat Engn Res, Xiamen 361005, Peoples R China

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2016年 / 66卷

基金：

中国国家自然科学基金;

关键词：

Acid-soluble collagen; LC-MS/MS; Characteristics; Tilapia scales; Fugu skin; PEPSIN-SOLUBILIZED COLLAGEN; CONVERTING-ENZYME; SWIM BLADDERS;

D O I：

10.1016/j.lwt.2015.10.070

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Acid-soluble collagen (ASC) was extracted from scales and skin of tilapia. The yields of scale collagen (SCC) and skin collagen (SKC) were 3.2 g/100 g and 27.2 g/100 g respectively, dry weight. The SDS-PAGE profile showed all collagen samples contained [alpha 1(I)](2)alpha 2(I) component and were characterized as type I collagen. LC-MS/MS analysis confirmed the SCC and SKC to be Oreochromis niloticus type I collagen. Amino acid analysis revealed the imino acid content from the SCC and SKC samples were 207 and 197 residues/1000 amino acid residues, respectively. Based on FTIR spectra and X-ray diffraction, isolation of ASC did not affect its three dimensional structure. Zeta potential studies indicated both SCC and SKC exhibited a net zero charge at pH 6.82 and 6.42, respectively. Both ASC samples exhibited high solubility in acidic pH (1-3) and lost their solubility at NaCl concentrations below 3 g/100 mL. The data collected here indicate that SCC and SKC may be suitable for use as an alternative to land-based mammalian collagen in food, nutraceuticals and pharmaceutical industries. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：453 / 459

页数：7

共 30 条

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