Chemical Constituents of Aerial Parts and Roots of Pycnanthemum flexuosum

An extract of whole plants of Pycnanthemum flexuosum showed an inhibitory effect on hyaluronidase activity. From an 80% acetone extract of aerial parts, 3-[(3E)-4-phenylbut-3-enoylamino]propionic acid, 3-O-beta-D-glucuronopyranosyl-echinocystic acid 28-O-beta-D-xylopyranosyl-(1 -> 3)-[3,4-diacetyl-beta-D-xylopyranosyl-(1 -> 4)]-alpha-L-rhamnopyranosyl-(1 -> 2)-alpha-L-arabinopyranosyl ester, vanillic acid 1-O-[(5-O-syringoyl)-beta-D-apiofuranosyl]-(1 -> 2)-beta-D-glucopyranoside, and (4S,5R)-4-hydroxy-5-phenyl-tetrahydrofuran-2-one were isolated together with 30 known compounds. Six known compounds were isolated from an 80% acetone extract of roots, and eritrichin was revealed as a hyaluronidase inhibitor in P. flexuosum.