Development and validation of an analytical method by HPLC for the simultaneous determination of ibuprofen and tiocolchicoside in tablets

Is was developed and validated an analytical methodology by HPLC for the simultaneous determination of Ibuprofen and Thiocolchicoside in tablets. The optimal separation conditions were achieved with the Hamilton PRP-1 column (5 mu m, 4.1 x 150 mm) with a mobile phase constituted by methanol: acetonitrile: water (10:20:70)% v / v y 0.05% triethylamine a pH adjustment to 8.0 +/- 0.1 with 85% orthophosphoric acid. The mobile phase flow was 1.3 mL / min, an injection volume of 5 mu L and the wavelength of 260 nm. The methodology presented linearity in the concentration range of 3.0 to 9.0 mg / mL for Ibuprofen and 0.02 to 0.06 mg / mL for Thiocolchicoside, with a coefficient of determination greater than 0.999, accuracy was obtained with a recovery percentage of 99.3 and 99.8% for Ibuprofen and 99.3 and 100.5% for Thiocolchicoside. The presence of degradation products was determined through the analysis of spectral purity, especially when the samples are exposed for 3 days in the presence hydrogen peroxide, and in Ibuprofen in the presence of acid hydrolysis. In the analysis of an expired drug it was demonstrated how the proposed method is able to determine the quantities of active principles present.