Analysis of hydroxylated polycyclic aromatic hydrocarbons in urine using comprehensive two-dimensional gas chromatography with a flame ionization detector

被引：31

作者：

Andre Amorim, Leiliane Coelho ^{[1
]}

Dimandja, Jean-Marie ^{[2
]}

Cardeal, Zenilda de Lourdes ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Chem, ICEx, BR-31270901 Belo Horizonte, MG, Brazil

[2] Spelman Coll, Dept Chem, Atlanta, GA 30314 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 14期

关键词：

GC x GC-FID; Hydroxy-PAH; Urine analysis; SOLID-PHASE MICROEXTRACTION; MASS-SPECTROMETRY; OCCUPATIONAL-EXPOSURE; LIQUID-EXTRACTION; DIESEL EXHAUST; PAH EXPOSURE; WORKERS; METABOLITES; 1-HYDROXYPYRENE; PHENANTHRENE;

D O I：

10.1016/j.chroma.2008.11.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of polycyclic aromatic hydrocarbon (PAH) metabolites in human urine is the method of choice for assessing exposure to carcinogenic compounds. The objective of this study was the development of a comprehensive two-dimensional gas chromatography (GC x CC) method using a flame ionisation detector (FID) to simultaneously determine 10 hydroxylated PAH. The method was based on enzymatic deconjugation, liquid-liquid extraction, and trimethylsilyl (TMS) derivatization of the analytes by microwave heating. Satisfactory separation was achieved. The coefficient of variance was 3.8-12.8%. LOD was 0.03-0.18 mu g/L, and LOQ was 0.1-0.5 mu g/L. The mean recovery was 76%. The method was applied to the analysis of urine from smokers and non-smokers. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：2900 / 2904

页数：5

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