Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection

被引：7
|
作者：
Cai, Jia [1 ,2 ,3 ]
Yang, Lin [1 ,2 ,3 ]
Wang, Bei [1 ,2 ,3 ]
Huang, Yucong [1 ,2 ,3 ]
Tang, Jufen [1 ,2 ,3 ]
Lu, Yishan [1 ,2 ,3 ]
Wu, Zaohe [2 ,3 ,4 ]
Jian, Jichang [1 ,2 ,3 ]
机构：
[1] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China
[3] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China
[4] Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2014年 / 36卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Ctenopharyngodon idella; N4BP1; Grass carp reovirus (GCRV); Viral gene; Viral invasion; UBIQUITIN LIGASES; PROMOTER ACTIVITY; KH DOMAINS; REOVIRUS; PROTEIN; ASSOCIATION; SUSCEPTIBILITY/RESISTANCE; RESISTANCE/SUSCEPTIBILITY; EXPRESSION; BINDING;
D O I：
10.1016/j.fsi.2013.11.003
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Nedd4 binding protein 1 (N4BP1) has been identified as an interacting protein and a substrate of Nedd4 E3 ligase. However, the report about N4BP1's function is limit. In this study, a novel N4BP1 gene (CiN4BP1) was cloned from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiN4BP1 (3022 bp) included an open reading frame (ORF) of 2565 bp, which encoded a putative peptides of 854 amino acids containing one KH domain and one NYN domain. It was close homology (47% identify) to Oryzias latipes N4BP1. And mRNA expression of CiN4BP1 gene showed relatively high level in skin, gill, head kidney and spleen. After grass carp reovirus (GCRV) infection, CiN4BP1 was up-regulated in vivo and in vitro. Furthermore, overexpression of CiN4BP1 in CIK cells inhibited viral gene transcription. These data indicated that CiN4BP1 might play an important role in immune response to viral invasion. (C) 2013 Published by Elsevier Ltd.
引用
收藏
页码：223 / 228
页数：6
相关论文
共 50 条
  • [11] Dietary supplementation with nanoparticle CMCS-20a enhances the resistance to GCRV infection in grass carp (Ctenopharyngodon idella)
    Wang, Zhensheng
    Xu, Chuang
    Zhang, Yanqi
    Huo, Xingchen
    Su, Jianguo
    FISH & SHELLFISH IMMUNOLOGY, 2022, 127 : 572 - 584
  • [12] Identification, characterization and immunological response analysis of stimulator of interferon gene (STING) from grass carp Ctenopharyngodon idella
    Feng, Xiaoli
    Yang, Chunrong
    Zhang, Yixuan
    Peng, Limin
    Chen, Xiaohui
    Rao, Youliang
    Gu, Tianle
    Su, Jianguo
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2014, 45 (01): : 163 - 176
  • [13] Identification of multifunctionality of grass carp (Ctenopharyngodon idella) TBK1 during bacterial infection
    Yu, Hongyan
    Jia, Xuewen
    Pang, Yifan
    Niu, Huiqin
    Du, Biao
    Xu, Xiaoyan
    Li, Jiale
    FISH & SHELLFISH IMMUNOLOGY, 2023, 136
  • [14] Identification and functional analysis of the toll-like receptor 20.2 gene in grass carp, Ctenopharyngodon idella
    Huang, Wen-Ji
    Yang, Xiaomeng
    Shen, Yubang
    Xu, Xiao-Yan
    Li, Lisen
    Wang, Rongquan
    Li, Jiale
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2016, 65 : 91 - 97
  • [15] Identification and characterization of the TLR18 gene in grass carp (Ctenopharyngodon idella)
    Huang, Wen-Ji
    Shen, Yubang
    Xu, Xiao-Yan
    Hu, Mo-Yan
    Li, Jia-le
    FISH & SHELLFISH IMMUNOLOGY, 2015, 47 (02) : 681 - 688
  • [16] Grass carp ( Ctenopharyngodon idella ) SIRT3 enhances MAVS-mediated antiviral innate immunity in response to GCRV infection
    Zhu, Chunchun
    Liu, Wen
    Jia, Shuke
    Sun, Xueyi
    Wang, Zixuan
    Deng, Hongyan
    Bai, Yao
    Xiao, Wuhan
    Liu, Xing
    AQUACULTURE, 2024, 587
  • [17] Identification of immune genes in grass carp Ctenopharyngodon idella in response to infection of the parasitic copepod Sinergasilus major
    M. X. Chang
    P. Nie
    G. Y. Liu
    Y. Song
    Q. Gao
    Parasitology Research, 2005, 96 : 224 - 229
  • [18] Identification of immune genes in grass carp Ctenopharyngodon idella in response to infection of the parasitic copepod Sinergasilus major
    Chang, MX
    Nie, P
    Liu, GY
    Song, Y
    Gao, Q
    PARASITOLOGY RESEARCH, 2005, 96 (04) : 224 - 229
  • [19] Cloning and characterization of the LEF/TCF gene family in grass carp (Ctenopharyngodon idella) and their expression profiles in response to grass carp reovirus infection
    Zhu, Denghui
    Huang, Rong
    Chen, Liangming
    Fu, Peipei
    Luo, Lifei
    He, Libo
    Li, Yongming
    Liao, Lanjie
    Zhu, Zuoyan
    Wang, Yaping
    FISH & SHELLFISH IMMUNOLOGY, 2019, 86 : 335 - 346
  • [20] Genome-wide identification and evolution of interleukins and their potential roles in response to GCRV and Aeromonas hydrophila challenge in grass carp (Ctenopharyngodon idella)
    Xu, Tianbing
    Wang, Zhensheng
    Gao, Yang
    Su, Jianguo
    AQUACULTURE, 2022, 556
12345