Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection

被引：8

作者：

Cai, Jia ^{[1
,2
,3
]}

Yang, Lin ^{[1
,2
,3
]}

Wang, Bei ^{[1
,2
,3
]}

Huang, Yucong ^{[1
,2
,3
]}

Tang, Jufen ^{[1
,2
,3
]}

Lu, Yishan ^{[1
,2
,3
]}

Wu, Zaohe ^{[2
,3
,4
]}

Jian, Jichang ^{[1
,2
,3
]}

机构：

[1] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Guangdong, Peoples R China

[2] Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

[3] Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China

[4] Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2014年 / 36卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Ctenopharyngodon idella; N4BP1; Grass carp reovirus (GCRV); Viral gene; Viral invasion; UBIQUITIN LIGASES; PROMOTER ACTIVITY; KH DOMAINS; REOVIRUS; PROTEIN; ASSOCIATION; SUSCEPTIBILITY/RESISTANCE; RESISTANCE/SUSCEPTIBILITY; EXPRESSION; BINDING;

D O I：

10.1016/j.fsi.2013.11.003

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Nedd4 binding protein 1 (N4BP1) has been identified as an interacting protein and a substrate of Nedd4 E3 ligase. However, the report about N4BP1's function is limit. In this study, a novel N4BP1 gene (CiN4BP1) was cloned from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiN4BP1 (3022 bp) included an open reading frame (ORF) of 2565 bp, which encoded a putative peptides of 854 amino acids containing one KH domain and one NYN domain. It was close homology (47% identify) to Oryzias latipes N4BP1. And mRNA expression of CiN4BP1 gene showed relatively high level in skin, gill, head kidney and spleen. After grass carp reovirus (GCRV) infection, CiN4BP1 was up-regulated in vivo and in vitro. Furthermore, overexpression of CiN4BP1 in CIK cells inhibited viral gene transcription. These data indicated that CiN4BP1 might play an important role in immune response to viral invasion. (C) 2013 Published by Elsevier Ltd.

引用

页码：223 / 228

页数：6

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