Objective: The contemporary trend in the field of autologous blood donation is quality assurance and improvement. Since the beginning of the decade some departments for transfusion medicine have tested the possibility of autologous hemapheresis. This paper reports about the suitability of the PES2 program (separator MCS 3p, Fa. Haemonetics) for the autologous blood donation. Material and Methods: PES2 program, cell separator MCS 3p, production of one unit erythrocyte concentrate (bca, SAG-M) and one unit fresh frozen plasma, examination of 314 donations for parameters of the machine program, the clinical side effects and the storage (days 0, 14, 35) of the blood products. Results: The clinical compatibility of the protocoll is especially good. The clinical side effects were 2.28%. The parameters of hemoglobin (11.57 mmol/l) and hematocrit (61.9%) at day 0 are comparable with those of conventionally manufactured blood products. The leukocyte contamination of the erythrocyte concentrates at day 0 (1.24 x 10(9)/blood unit) was in the range of the critical load. The platelets amount on day 0 to 32.92 Gpt/1; this corresponds with a concentration in the erythrocyte concentrate of 9.21 x 10(9) At the end of the storage of these units (day 35) the hemolysis rate was 0.51%. Conclusions: The very good compatibility of the erythroplasmapheresis, the easy use and the good quality of the manufactured blood products recommend the PES2 program for further use in departments of transfusion medicine and for hospitals which want to build up their own autologous blood donation program.