The pathogenesis of Newcastle disease: A comparison of selected Newcastle disease virus wild-type strains and their infectious clones

被引：46

作者：

Wakamatsu, Nobuko

King, Daniel J.

Seal, Bruce S.

Samal, Siba K.

Brown, Corrie C.

机构：

[1] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA

[2] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA 30605 USA

[3] USDA ARS, Poultry Microbiol Safety Unit, Russell Res Ctr, Athens, GA 30605 USA

[4] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA

来源：

VIROLOGY | 2006年 / 353卷 / 02期

关键词：

fusion protein; hemagglutinin-neuraminidase protein; immunohistochemistry; in situ hybridization; phosphoprotein; reverse genetics; avian paramyxovirus; veterinary virology; emerging disease;

D O I：

10.1016/j.virol.2006.06.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effect of mutations of Newcastle disease virus (NDV) fusion (F) gene, hemagglutinin-neuraminidase (HN) gene, and phosphoprotein (P) gene and HN chimeras between the virulent Beaudette C and low virulence LaSota strains on pathogenesis and pathogenicity was examined in fully susceptible chickens. A virulent F cleavage site motif within a LaSota backbone increased pathogenicity and severity of clinical disease. A LaSota HN within a Beaudette C backbone decreased pathogenicity indices and disease severity. A Beaudette C HN within a LaSota backbone did not change either pathogenicity indices or severity of disease in chickens. Loss of glycosylation at site 4 of the HN or modified P gene of Beaudette C decreased pathogenicity indices and caused no overt clinicopathologic disease in chickens. Both pathogenicity indices and clinicopathologic examination demonstrated that the F, RN, and P genes of NDV collectively or individually can contribute to viral virulence. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：333 / 343

页数：11

共 41 条

[1] Tissue tropism in the chicken embryo of non-virulent and virulent Newcastle diseases strains that express green fluorescence protein
Al-Garib, SO
Gielkens, ALJ
Gruys, E
Peeters, BPH
Koch, G
[J]. AVIAN PATHOLOGY, 2003, 32 (06) : 591 - 596
[2] Newcastle disease
Alexander, D
[J]. BRITISH POULTRY SCIENCE, 2001, 42 (01) : 5 - 22
[3] Alexander D.J., 1998, A laboratory manual for the isolation and identification of avian pathogens, V4th, P156
[4] NEWCASTLE-DISEASE IN COUNTRIES OF THE EUROPEAN UNION
ALEXANDER, DJ
[J]. AVIAN PATHOLOGY, 1995, 24 (01) : 3 - 10
[5] Alexander DJ, 2003, DIS POULTRY, P63
[6] Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence
Brown, C
King, DJ
Seal, BS
[J]. VETERINARY PATHOLOGY, 1999, 36 (02) : 125 - 132
[7] MOLECULAR-CLONING OF COMPLEMENTARY-DNA TO NEWCASTLE-DISEASE VIRUS, AND NUCLEOTIDE-SEQUENCE ANALYSIS OF THE JUNCTION BETWEEN THE GENES ENCODING THE HEMAGGLUTININ NEURAMINIDASE AND THE LARGE PROTEIN
CHAMBERS, P
MILLAR, NS
BINGHAM, RW
EMMERSON, PT
[J]. JOURNAL OF GENERAL VIROLOGY, 1986, 67 : 475 - 486
[8] Complete nucleotide sequence of Newcastle disease virus:: evidence for the existence of a new genus within the subfamily Paramyxovirinae
de Leeuw, O
Peeters, B
[J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 131 - 136
[9] Virulence of Newcastle diseasevirus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein
de Leeuw, OS
Koch, G
Hartog, L
Ravenshorst, N
Peeters, BPH
[J]. JOURNAL OF GENERAL VIROLOGY, 2005, 86 : 1759 - 1769
[10] Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain
de Leeuw, OS
Hartog, L
Koch, G
Peeters, BPH
[J]. JOURNAL OF GENERAL VIROLOGY, 2003, 84 : 475 - 484

← 1 2 3 4 5 →