Pharmacokinetics of Araloside X in Rats and Tissue Distribution in Mice by UPLC-MS/MS

An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantification of araloside X which was then applied in pharmacokinetics study in rat and tissue distribution in mouse. Six male Sprague-Dawley rats were used for pharmacokinetics after intravenous (8 mg/kg) administration of araloside X. Thirty mice were randomly divided into six groups (five mice for each group, one group for each time point) and received 16 mg/kg of araloside X by intraperitoneal administration. The linear range of calibration curve was over 10-2000 ng/mL for araloside X in rat plasma and mouse tissues. The results showed that araloside X was distributed in the liver, kidney, spleen and lung of mice. According to the concentration of each tissue, the distribution of drug-time histogram was drawn to better understand the metabolism of araloside X in tissues, and provide a scientific basis for the broad pharmacodynamic basis of araloside X.