Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly

被引：81

作者：

Cui, Ying ^{[1
,2
,3
]}

Fan, Shanji ^{[6
]}

Yuan, Ze ^{[1
,2
]}

Song, Minghui ^{[1
,2
]}

Hu, Jiawen ^{[1
,2
]}

Qian, Dong ^{[5
]}

Zhen, Deshuai ^{[1
,2
]}

Li, Junhua ^{[3
]}

Zhu, Baode ^{[4
]}

机构：

[1] Hunan Univ, Hunan Key Lab Two Dimens Mat, Adv Catalyt Engn Res Ctr, Minist Educ, Changsha 410082, Hunan, Peoples R China

[2] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[3] Hengyang Normal Univ, Coll Chem & Mat Sci, Hengyang 421001, Peoples R China

[4] Xiangnan Univ, Coll Chem Biol & Environm Engn, Chenzhou 423043, Peoples R China

[5] Cent South Univ, Coll Chem & Chem Engn, Hunan Prov Key Lab Chem Power Resources, Changsha 410083, Peoples R China

[6] Univ South China, Dept Thyroid Breast Surg, Affiliated Hosp 1, Hengyang 421001, Peoples R China

来源：

TALANTA | 2021年 / 224卷

关键词：

Electrochemical biosensor; CRISPR-Cas13a system; Catalytic hairpin assembly; Cascade signal amplification; miRNA-21; NUCLEIC-ACID DETECTION; DETECTION PLATFORM; AMPLIFICATION; DIAGNOSTICS; BIOSENSOR; SYSTEM;

D O I：

10.1016/j.talanta.2020.121878

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are related to many biological processes and regarded as biomarkers of disease. Rapid, sensitive, and specific methods for miRNA assay are very important for early disease diagnostic and therapy. In the present work, an ultrasensitive electrochemical biosensing platform has been developed for miRNA-21 assay by combining CRISPR-Cas13a system and catalytic hairpin assembly (CHA). In the presence of miRNA-21, it would hybridize with the spacer region of Cas13a/crRNA duplex to activate the cleavage activity of CRISPR-Cas13a system, leading to the release of initiator of CHA to generate amplified electrochemical signals. Base on the CRISPR-Cas13a-mediated cascade signal amplification strategy, the developed electrochemical biosensing platform exhibited high sensitivity with a low detection limit of 2.6 fM (S/N = 3), indicating that the platform has great potential for application in early clinical diagnostic.

引用

页数：7

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