Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates

被引:4
作者
Dou, Horng-Yunn [1 ]
Lu, Jang-Jih [2 ,3 ,4 ]
Lin, Chih-Wei [1 ]
Chang, Jia-Ru [1 ]
Sun, Jun-Ren [2 ,3 ]
Su, Ih-Jen [1 ]
机构
[1] Natl Hlth Res Inst, Div Clin Res, Zhunan 350, Miaoli County, Taiwan
[2] Tri Serv Gen Hosp, Dept Pathol, Div Clin Pathol, Taipei 114, Taiwan
[3] Natl Def Med Ctr, Taipei 114, Taiwan
[4] China Med Univ Hosp, Dept Lab Med, Taichung 404, Taiwan
关键词
Mycobacterium tuberculosis; MIRU-VNTR typing; INTERSPERSED REPETITIVE UNITS; MOLECULAR EPIDEMIOLOGY; DISCRIMINATORY ABILITY; CLINICAL-SAMPLES; BEIJING FAMILY; COMPLEX; DIVERSITY; STRAINS; IS6110;
D O I
10.1016/j.mimet.2009.01.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian and U lineages. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:127 / 129
页数:3
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