Human dermal fibroblasts express eotaxin: Molecular cloning, mRNA expression, and identification of eotaxin sequence variants

被引：96

作者：

Bartels, J ^{[1
]}

Schluter, C ^{[1
]}

Richter, E ^{[1
]}

Noso, N ^{[1
]}

Kulke, R ^{[1
]}

Christophers, E ^{[1
]}

Schroder, JM ^{[1
]}

机构：

[1] FORSCHUNGSINST BORSTEL,DEPT IMMUNOL & CELL BIOL,D-23845 BORSTEL,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 225卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1292

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently we discovered and purified a novel beta-chemokine with eosinophil specific chemotactic activity from supematants of long-term TNF-alpha stimulated dermal fibroblasts. Using degenerated specific oligonucleotides based on partial amino acid sequence data and a PCR protocol, we obtained different clones sharing high sequence homology with this novel chemokine and with human eotaxin cDNA. Semi-quantitative RT-PCR experiments using eotaxin-specific primers indicate low constitutive eotaxin mRNA expression in human dermal fibroblasts which is upregulated by LL-1 alpha and TNF-alpha within 6 hrs and modulated by IFN-gamma. While IL-1 alpha-induced eotaxin mRNA accumulation is transient, long-term stimulation with TNF-alpha resulted in a further increase of eotaxin mRNA. (C) 1996 Academic Press, Inc.

引用

页码：1045 / 1051

页数：7

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