Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1

被引:32
作者
Cao, Xiao-Hong [1 ]
Liao, Zhen-Yu [1 ]
Wang, Chun-Ling [1 ]
Cai, Ping [1 ]
Yang, Wen-Yan [2 ]
Lu, Mei-Fang [1 ]
Huang, Guo-Wei [3 ]
机构
[1] Tianjin Univ Sci & Technol, Sch Food Sci & Biotechnol, Tianjin 300457, Peoples R China
[2] Tianjin Univ Sci & Technol, Sch Marine Sci & Engn, Tianjin 300457, Peoples R China
[3] Tianjin Med Univ, Sch Publ Hlth, Tianjin 300052, Peoples R China
基金
中国国家自然科学基金;
关键词
antitumour activity; apoptosis; Bacillus natto TK-1; caspase; 3; lactate dehydrogenase (LDH); lipopeptide biosurfactant; CELL-CYCLE ARREST; INDUCED APOPTOSIS; SURFACTIN; DRUG; DIFFERENTIATION; INACTIVATION; CULTURE;
D O I
10.1042/BA20070227
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.
引用
收藏
页码:97 / 106
页数:10
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