Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1

被引：32

作者：

Cao, Xiao-Hong ^{[1
]}

Liao, Zhen-Yu ^{[1
]}

Wang, Chun-Ling ^{[1
]}

Cai, Ping ^{[1
]}

Yang, Wen-Yan ^{[2
]}

Lu, Mei-Fang ^{[1
]}

Huang, Guo-Wei ^{[3
]}

机构：

[1] Tianjin Univ Sci & Technol, Sch Food Sci & Biotechnol, Tianjin 300457, Peoples R China

[2] Tianjin Univ Sci & Technol, Sch Marine Sci & Engn, Tianjin 300457, Peoples R China

[3] Tianjin Med Univ, Sch Publ Hlth, Tianjin 300052, Peoples R China

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2009年 / 52卷

基金：

中国国家自然科学基金;

关键词：

antitumour activity; apoptosis; Bacillus natto TK-1; caspase; 3; lactate dehydrogenase (LDH); lipopeptide biosurfactant; CELL-CYCLE ARREST; INDUCED APOPTOSIS; SURFACTIN; DRUG; DIFFERENTIATION; INACTIVATION; CULTURE;

D O I：

10.1042/BA20070227

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.

引用

页码：97 / 106

页数：10

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