hCTLA4-gene modified human bone marrow - Derived mesenchymal stem cells as allogeneic seed cells in bone tissue engineering

被引：16

作者：

Dai, Fei

Shi, Dongwen

He, Weifeng

Wu, Jun ^{[1
]}

Luo, Gaoxin

Yi, Shaoxuan

Xu, Jianzhong

Chen, Xiwei

机构：

[1] SW Hosp, Inst Burn Res, Chongqing 400038, Peoples R China

[2] SW Hosp, Dept Orthopaed, Chongqing 400038, Peoples R China

[3] Chongqing Key Lab Proteom Dis, Chongqing, Peoples R China

来源：

TISSUE ENGINEERING | 2006年 / 12卷 / 09期

关键词：

D O I：

10.1089/ten.2006.12.2583

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Immune rejection of heterogeneic seed cells, even of low antigenicity human bone marrow derived mesenchymal stem cells (hBMMSCs), remains an obstacle for bone tissue engineering. It is still a question how to transfer the gene of human cytotoxic T lymphocyte-associated antigen 4-Ig (CTLA4-Ig), a well-known ligand for CD80/CD86, to hBMMSCs to prolong survival time while not interfering with their osteogenesis in vivo. Our data, for the first time, clearly demonstrate that CTLA4-Ig gene modified hBMMSCs could normally differentiate to osteoblasts in vitro, significantly inhibit immune response in the model of mixed lymphocyte culture, and survive until bone tissue formed in vivo. Thus, CTLA4Ig gene-modified hBMMSCs might be promising seed cells for bone tissue engineering.

引用

页码：2583 / 2590

页数：8

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