New medium composition for high betacyanin production by a cell suspension culture of table beet (Beta vulgaris L.)

被引：17

作者：

Akita, T ^{[1
]}

Hina, Y ^{[1
]}

Nishi, T ^{[1
]}

机构：

[1] Nippon Shinyaku Inst Bot Res, Yamashina Ku, Kyoto 6078182, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 04期

关键词：

Beta vulgaris L; betacyanin; cell suspension culture; culture medium;

D O I：

10.1271/bbb.66.902

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of a revised Linsmaier-Skoog (LS) medium on betacyanin production was investigated in suspension cultures of table beet (Beta vulgaris L.). The effects of a high iron concentration and low concentration of zinc on betacyanin production were not cumulative. The composition of the new revised medium for high betacyanin production was established by reducing the concentration of inorganic nitrogen (30 mM), modifying the ratio of ammonium to nitrate (1:14), reducing the concentration of zinc (0.0003 mM), and removing copper and cobalt. The revised LS medium enabled the maximum betacyanin yield of 550 mg/l to be obtained from a 14-day culture. This medium promoted the betacyanin production in three types of cell line differing in the betacyanin productivity. The betacyanin productivity (40 mg/l.day) was higher than that quoted in any other previous reports.

引用

页码：902 / 905

页数：4

共 13 条

[1] Production of betacyanins by a cell suspension culture of table beet (Beta vulgaris L.) [J].

Akita, T ;

Hina, Y ;

Nishi, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2000, 64 (09) :1807-1812

[2] Effect of zinc deficiency on betacyanin production in a cell suspension culture table beet (Beta vulgaris L.) [J].

Akita, T ;

Hina, Y ;

Nishi, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (04) :962-965

[3] CAUSE OF DIFFERENTIAL SUSCEPTIBILITY TO ZINC DEFICIENCY IN 2 VARIETIES OF NAVY BEANS (PHASEOLUS VULGARIS L) [J].

AMBLER, JE ;

BROWN, JC .

AGRONOMY JOURNAL, 1969, 61 (01) :41-&

[4] PRODUCTION OF BETALAINS BY SUSPENSION-CULTURES OF CHENOPODIUM-RUBRUM L [J].

BERLIN, J ;

SIEG, S ;

STRACK, D ;

BOKERN, M ;

HARMS, H .

PLANT CELL TISSUE AND ORGAN CULTURE, 1986, 5 (03) :163-174

[5] SECONDARY METABOLISM IN CULTURED RED BEET (BETA-VULGARIS L) CELLS - DIFFERENTIAL REGULATION OF BETAXANTHIN AND BETACYANIN BIOSYNTHESIS [J].

GIROD, PA ;

ZRYD, JP .

PLANT CELL TISSUE AND ORGAN CULTURE, 1991, 25 (01) :1-12

[6]

Jiménez-Aparicio A, 1999, ADV EXP MED BIOL, V464, P195

[7]

LEATHERS RR, 1992, IN VITRO CELL DEV-PL, V28P, P39

[8] ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES [J].

LINSMAIER, EM ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1965, 18 (01) :100-+

[9] EFFECT OF CARBON SOURCE ON CELL-GROWTH AND BETALAIN PRODUCTION IN CELL-SUSPENSION CULTURE OF BETA-VULGARIS [J].

MONROY, MR ;

APARICIO, AJ ;

ORTIZ, GD ;

JIMENEZ, GS .

BIOTECHNOLOGY LETTERS, 1994, 16 (08) :853-858

[10]

ROSELL RAMON A., 1964, SOIL SCI, V97, P152, DOI 10.1097/00010694-196403000-00002

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