Molecular crowding effects on protein stability

被引：48
作者：
Despa, Florin
Orgill, Dennis P.
Lee, Raphael C.
机构：
[1] Univ Chicago, Dept Surg, Chicago, IL 60637 USA
[2] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Surg, Boston, MA 02115 USA
来源：
CELL INJURY: MECHANISMS, RESPONSES, AND REPAIR | 2005年 / 1066卷
关键词：
crowding effects; protein denaturation; thermal injury;
D O I：
10.1196/annals.1363.005
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The volume fraction occupied by the dry matter of the cell can be as large as 40%, of which more than half (-60%) are proteins. Thus, cellular proteins and protein assemblies occupy a large volume that can have a profound effect on their own native-state stabilities and on their unfolding/refolding rates. In addition, macromolecular crowding can change the properties of a significant fraction of the water in the cell. We review features of the molecular crowding effect which are relevant for describing the microscopic mechanism of thermal injuries.
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页码：54 / 66
页数：13
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