Lane tracking software for four-color fluorescence-based electrophoretic gel images

被引：7

作者：

Cooper, ML ^{[1
]}

Maffitt, DR ^{[1
]}

Parsons, JD ^{[1
]}

Hillier, L ^{[1
]}

States, DJ ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,INST BIOMED COMP,ST LOUIS,MO 63110

来源：

GENOME RESEARCH | 1996年 / 6卷 / 11期

关键词：

D O I：

10.1101/gr.6.11.1110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Software to track sample lanes automatically in four-color, fluorescence-based, electrophoretic gel images has been developed for application in large-scale DNA sequencing projects. Lanes and lane boundaries are tracked by analyzing a first difference approximation to the gradient of a vertically integrated and processed ''brightness'' profile. Initially lanes are located in a region of the gel image selected for good horizontal lane spacing and signal strength. The software uses models of expected lane and interlane spacing and lateral lane behavior to maintain accurate tracking on imperfect gels. In areas where intensity-based tracking is difficult, interpixel column correlation is also used to locate and define lane features. Summary statistics and compressed-in-time images are generated for user evaluation of tracking performance. The software developed has been tested successfully on gel images with degradations including significant horizontal lane motion (curving) and image artifacts, and is now in full-scale use in our sequencing projects.

引用

页码：1110 / 1117

页数：8

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