A new transcriptional activator for amylase genes in Aspergillus

被引：97
|
作者：
Petersen, KL [1 ]
Lehmbeck, J [1 ]
Christensen, T [1 ]
机构：
[1] Novo Nordisk AS, Dept Fungal Genet, DK-2880 Bagsvaerd, Denmark
来源：
MOLECULAR AND GENERAL GENETICS | 1999年 / 262卷 / 4-5期
关键词：
amyR; amylases; gene regulation; Aspergillus oryzae;
D O I：
10.1007/s004380051129
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned a regulatory gene for amylase synthesis in Aspergillus oryzae. This gene, amyR, encodes a 604-amino acid transcriptional activator with a Cys(6) zinc cluster, that shows extensive homology to the DNA binding domain of GAL4 from Saccharomyces cerevisiae. The DNA binding domain of amyR binds to two types of sequences found in a number of promoters from Aspergillus genes coding for starch-degrading enzymes. One type of binding site is characterized by two CGG triplets separated by eight nucleotides. The other type has only one CGG triplet, which is followed by the sequence AAATTTAA.
引用
收藏
页码：668 / 676
页数：9
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