Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil

被引：16

作者：

Vieira de Melo, Cynthia Farias ^{[1
,2
]}

Gigek, Carolina Oliveira ^{[4
]}

da Silva, Juarez Nobrega ^{[1
,2
]}

Cardoso Smith, Marilia de Arruda ^{[4
]}

de Araujo, Rubistenia Miranda ^{[1
,2
]}

Burbano, Rommel Rodriguez ^{[5
]}

Lima, Eleonidas Moura ^{[3
]}

机构：

[1] Univ Fed Paraiba, Grad Program Cellular & Mol Biol, Dept Mol Biol, BR-58051900 Joao Pessoa, Paraiba, Brazil

[2] Univ Fed Paraiba, Lab Struct Mol Biol & Oncogenet LBMEO, Dept Mol Biol, BR-58051900 Joao Pessoa, Paraiba, Brazil

[3] Univ Fed Paraiba, CCEN, Dept Mol Biol, Lab Struct Mol Biol & Oncogenet LBMEO, BR-58051900 Joao Pessoa, Paraiba, Brazil

[4] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04023900 Sao Paulo, Brazil

[5] Fed Univ Para, Ctr Biol Sci, Dept Biol, BR-66075110 Belem, Para, Brazil

来源：

TUMOR BIOLOGY | 2014年 / 35卷 / 02期

关键词：

Gastric cancer; Methylation; COX2; PROMOTER HYPERMETHYLATION; HELICOBACTER-PYLORI; GROWTH-FACTOR; EXPRESSION; CYCLOOXYGENASE-2; METHYLATION; INDIVIDUALS; MATRIX; CDKN2A; ISLAND;

D O I：

10.1007/s13277-013-1148-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To verify the methylation status of THBS1, GPX3, and COX2 genes and to evaluate their association with Helicobacter pylori in gastric adenocarcinomas. Methylation-sensitive restriction enzyme PCR assay was performed in 16 diffuse type gastric cancer samples, 23 intestinal type, and 15 normal stomach tissue. The presence of H. pylori was performed by amplification of the fragment of the 16S rRNA. Statistical analyses were performed using Fisher's exact test. The hypermethylation of GPX3, THBS1, and COX2 occurred in 18 (n = 7), 5 (n = 2), and 36 % (n = 14) of gastric cancer samples, respectively, whereas in normal samples, it was found in 13, 7, and 67 %. The presence of H. pylori was detected in 67 % of gastric cancer samples and 67 % in normal gastric samples. The methylation of THBS1 and GPX3 was not significantly different between the types of tumors, normal sample, the presence of H. pylori, or clinicopathological variables studied (P > 0.05). However, the methylation status of the gene COX2 is significantly different between normal tissue and intestinal type gastric cancer (P = 0.02). Therefore, our results suggest that the methylation status of the gene COX2 is associated with the intestinal type of gastric cancer.

引用

页码：1107 / 1111

页数：5

共 32 条

[21] PLATELET-DERIVED GROWTH-FACTOR AND HEPARIN-LIKE GLYCOSAMINOGLYCANS REGULATE THROMBOSPONDIN SYNTHESIS AND DEPOSITION IN THE MATRIX BY SMOOTH-MUSCLE CELLS [J].