The Validity of Marker-assisted Selection Using DNA Markers Linked to a Pear Scab Resistance Gene (Vnk) in Two Populations

被引：9

作者：

Gonai, Takeru ^{[1
]}

Terakami, Shingo ^{[2
]}

Nishitani, Chikako ^{[2
]}

Yamamoto, Toshiya ^{[2
]}

Kasumi, Masakazu ^{[1
]}

机构：

[1] Ibaraki Agr Ctr, Inst Plant Biotechnol, Kasama, Ibaraki 3190292, Japan

[2] Natl Inst Fruit Tree Sci, Tsukuba, Ibaraki 3058605, Japan

来源：

JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE | 2009年 / 78卷 / 01期

关键词：

Japanese pear; 'Kinchaku'; marker-assisted selection; Venturia nashicola; scab resistance; BLACK SPOT DISEASE; JAPANESE; INHERITANCE; MAP; VF;

D O I：

10.2503/jjshs1.78.49

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Marker-assisted selection (MAS) using 5 DNA markers linked to the pear scab resistance derived from 'Kinchaku' (Vnk) was examined in a pear breeding program. The relationship between DNA markers and scab resistance was investigated. All individuals without both DNA markers of STS-OPW2 and STS-OPO9 derived from 'Kinchaku' showed scab symptoms with heavy sporulation on leaves. Individuals possessing these markers exhibited no symptoms, or necrosis with or without weak sporulation on petioles which was localized and did not affect growth, and were classified as resistant. Hence, elimination of individuals that have neither STS-OPW2 nor STS-OPO9 would be a suitable strategy to select for scab resistance in Japanese pear breeding when introgressing Vnk from 'Kinchaku'.

引用

页码：49 / 54

页数：6

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