Histocompatibility reaction in tissue and cells of the marine sponge Suberites domuncula in vitro and in vivo:: central role of the allograft inflammatory factor 1

被引:44
作者
Müller, WEG
Krasko, A
Skorokhod, A
Bünz, C
Grebenjuk, VA
Steffen, R
Batel, R
Schröder, HC
机构
[1] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany
[2] Rudjer Boskovic Inst, Ctr Marine Res, Rovinj 52210, Croatia
关键词
sponges; autograft; allograft; mixed sponge cell reaction; Suberites domuncula;
D O I
10.1007/s00251-002-0441-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Sponges (Porifera) are the phylogenetically oldest still extant metazoan phylum. Recently elements of their immune system have been cloned and analyzed, primarily from the demosponges Suberites domuncula and Geodia cydonium. By differential display, two genes were identified in S. domuncula, whose translation products are involved in graft rejection/fusion: the allograft inflammatory factor (AIF-1) and the Tcf-like transcription factor (TCF). Since the AIF-1 and TCF genes are upregulated in vivo after tissue transplantation, especially in allografts, we investigated whether this reaction can be monitored in vitro. Therefore, the autogeneic and the allogeneic mixed sponge cell reaction (MSCR) system was applied for the first time to identify distinct factors in sponges in vitro. The results confirm that the two AIF-1 and TCF genes are induced during allogeneic MSCR. Furthermore, the recombinant sponge AIF-1 causes an upregulation of the expression of the TCF. We conclude that the AIF-1 and TCF genes are upregulated in sponges during histoincompatibility reactions; the data support the view that sponges have immune systems composed of highly complex elements related to those found in mammalian systems.
引用
收藏
页码:48 / 58
页数:11
相关论文
共 59 条
  • [1] Abbas A.K., 1994, Cellular and molecular immunology
  • [2] Cloning and expression of new receptors belonging to the immunoglobulin superfamily from the marine sponge Geodia cydonium
    Blumbach, B
    Diehl-Seifert, B
    Seack, J
    Steffen, R
    Müller, IM
    Müller, WEG
    [J]. IMMUNOGENETICS, 1999, 49 (09) : 751 - 763
  • [3] STRUCTURAL FEATURES OF THE HMG CHROMOSOMAL-PROTEINS AND THEIR GENES
    BUSTIN, M
    LEHN, DA
    LANDSMAN, D
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (03) : 231 - 243
  • [4] Cho EA, 1998, MECH DEVELOP, V77, P9
  • [5] COLIGAN JE, 2000, CURRENT PROTOCOLS PR
  • [6] GRAFT-REJECTION IN SPONGES - GENETIC-STRUCTURE OF ACCEPTING AND REJECTING POPULATIONS
    CURTIS, ASG
    KERR, J
    KNOWLTON, N
    [J]. TRANSPLANTATION, 1982, 33 (02) : 127 - 133
  • [7] Primmorphs generated from dissociated cells of the sponge Suberites domuncula:: a model system for studies of cell proliferation and cell death
    Custodio, MR
    Prokic, I
    Steffen, R
    Koziol, C
    Borojevic, R
    Brümmer, F
    Nickel, M
    Müller, WEG
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 1998, 105 (1-2) : 45 - 59
  • [8] Dayhoff M.O., 1978, Atlas of Protein Sequence and Structure, P345
  • [9] Du Pasquier L, 2000, CURR TOP MICROBIOL, V248, P159
  • [10] Duval A, 2000, CANCER RES, V60, P3872