METHODOLOGY VALIDATION FOR EVALUATION OF THE UNCERTAINTY IN THE CALIBRATION CURVES BETTER ADJUSTED FOR SECOND-DEGREE POLYNOMIALS.

METHODOLOGY VALIDATION FOR EVALUATION OF THE UNCERTAINTY IN THE CALIBRATION CURVES BETTER ADJUSTED FOR SECOND-DEGREE POLYNOMIALS. The most widespread literature for the evaluation of uncertainty - GUM and Eurachem - does not describe explicitly how to deal with uncertainty of the concentration coming from non-linear calibration curves. This work had the objective of describing and validating a methodology, as recommended by the recent GUM Supplement approach. to evaluate the uncertainty through polynomial models of the second order. In the uncertainty determination of the concentration of benzatone (C) by chromatography, it is observed that the uncertainty of measurement between the methodology proposed and Monte Carlo Simulation, does not diverge by more than 0.0005 Unit. thus validating the model proposed for one significant digit.