Proteomics Answers Which Yeast Genes Are Specific for Baking, Brewing, and Ethanol Production

被引：8

作者：

Davydenko, Svetlana ^{[1
]}

Meledina, Tatiana ^{[2
]}

Mittenberg, Alexey ^{[3
]}

Shabelnikov, Sergey ^{[3
]}

Vonsky, Maksim ^{[4
]}

Morozov, Artyom ^{[2
]}

机构：

[1] Baltika BreweriesPart Carlsberg Grp, Innovat & Res Dept, 6 Th Verkhnij Ln 3, St Petersburg 194292, Russia

[2] ITMO Univ, Fac Biotechnol BioTech, Lomonosova St 9, St Petersburg 191002, Russia

[3] Russian Acad Sci, Cell Technol Ctr, Inst Cytol, Prote & Mass Spectrometry Grp, Tikhoretsky Av 4, St Petersburg 194064, Russia

[4] DI Mendeleyev Inst Metrol VNIIM, Dept State Stand & Reference Mat Area Bioanalyt &, Moskovsky Pr 19, St Petersburg 190005, Russia

来源：

BIOENGINEERING-BASEL | 2020年 / 7卷 / 04期

关键词：

yeast; domestication; protein expression; brewing; baking; ethanol production; industrial strains;

D O I：

10.3390/bioengineering7040147

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeast strains are convenient models for studying domestication processes. The ability of yeast to ferment carbon sources from various substrates and to produce ethanol and carbon dioxide is the core of brewing, winemaking, and ethanol production technologies. The present study reveals the differences among yeast strains used in various industries. To understand this, we performed a proteomic study of industrial Saccharomyces cerevisiae strains followed by a comparative analysis of available yeast genetic data. Individual protein expression levels in domesticated strains from different industries indicated modulation resulting from response to technological environments. The innovative nature of this research was the discovery of genes overexpressed in yeast strains adapted to brewing, baking, and ethanol production, typical genes for specific domestication were found. We discovered a gene set typical for brewer's yeast strains. Baker's yeast had a specific gene adapted to osmotic stress. Toxic stress was typical for yeast used for ethanol production. The data obtained can be applied for targeted improvement of industrial strains.

引用

页码：1 / 15

页数：15

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