A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes

被引：114

作者：

Zhong, Fudi ^{[1
,2
,3
]}

Zhou, Nan ^{[1
,2
,3
]}

Wu, Kang ^{[1
,2
,3
]}

Guo, Yubiao ^{[4
,5
]}

Tan, Weiping ^{[6
]}

Zhang, Hong ^{[1
,2
,3
]}

Zhang, Xue ^{[1
,2
,3
]}

Geng, Guannan ^{[1
,2
,3
]}

Pan, Ting ^{[1
,2
,3
]}

Luo, Haihua ^{[1
,2
,3
]}

Zhang, Yijun ^{[1
,2
,3
]}

Xu, Zhibin ^{[1
,2
,3
]}

Liu, Jun ^{[1
,2
,3
]}

Liu, Bingfeng ^{[1
,2
,3
]}

Gao, Wenchao ^{[1
,2
,3
]}

Liu, Chao ^{[1
,2
,3
]}

Ren, Liangliang ^{[1
,2
,3
]}

Li, Jun ^{[1
,2
,3
]}

Zhou, Jie ^{[1
,2
,3
]}

Zhang, Hui ^{[1
,2
,3
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Sch Med, Key Lab Trop Dis Control, Minist Educ China, Guangzhou 510080, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Zhongshan Sch Med, Guangdong Engn Res Ctr Antimicrobial Agent & Immu, Guangzhou 510080, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 1, Div Resp, Guangzhou 510080, Guangdong, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 1, Med Intens Care Unit, Guangzhou 510080, Guangdong, Peoples R China

[6] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pediat, Guangzhou 510080, Guangdong, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 2015年 / 43卷 / 21期

关键词：

NOVO DNA METHYLATION; PIWI PROTEINS; DROSOPHILA-MELANOGASTER; CHROMATIN STATE; GENE-EXPRESSION; MOUSE GERMLINE; NONCODING RNA; CANCER-CELLS; 3' TERMINI; IDENTIFICATION;

D O I：

10.1093/nar/gkv954

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and are involved in maintaining genome integrity by silencing transposons. These are also involved in DNA/histone methylation and gene expression regulation in somatic cells of invertebrates. The functions of piRNAs in somatic cells of vertebrates, however, remain elusive. We found that snoRNA-derived and C (C')/D' (D)-box conserved piRNAs are abundant in human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to pre-mRNA intron, which subsequently inhibited the development of Th2 T-lymphocytes. Piwil4 and Ago4 are associated with this piRNA, and this complex further interacts with Trf4-Air2-Mtr4 Polyadenylation (TRAMP) complex, which leads to the decay of targeted pre-mRNA through nuclear exosomes. Taken together, we demonstrate a novel piRNA mechanism in regulating gene expression in highly differentiated somatic cells and a possible novel target for allergy therapeutics.

引用

页码：10474 / 10491

页数：18

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