A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes

被引：114

作者：

Zhong, Fudi ^{[1
,2
,3
]}

Zhou, Nan ^{[1
,2
,3
]}

Wu, Kang ^{[1
,2
,3
]}

Guo, Yubiao ^{[4
,5
]}

Tan, Weiping ^{[6
]}

Zhang, Hong ^{[1
,2
,3
]}

Zhang, Xue ^{[1
,2
,3
]}

Geng, Guannan ^{[1
,2
,3
]}

Pan, Ting ^{[1
,2
,3
]}

Luo, Haihua ^{[1
,2
,3
]}

Zhang, Yijun ^{[1
,2
,3
]}

Xu, Zhibin ^{[1
,2
,3
]}

Liu, Jun ^{[1
,2
,3
]}

Liu, Bingfeng ^{[1
,2
,3
]}

Gao, Wenchao ^{[1
,2
,3
]}

Liu, Chao ^{[1
,2
,3
]}

Ren, Liangliang ^{[1
,2
,3
]}

Li, Jun ^{[1
,2
,3
]}

Zhou, Jie ^{[1
,2
,3
]}

Zhang, Hui ^{[1
,2
,3
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Zhongshan Sch Med, Key Lab Trop Dis Control, Minist Educ China, Guangzhou 510080, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Zhongshan Sch Med, Guangdong Engn Res Ctr Antimicrobial Agent & Immu, Guangzhou 510080, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 1, Div Resp, Guangzhou 510080, Guangdong, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 1, Med Intens Care Unit, Guangzhou 510080, Guangdong, Peoples R China

[6] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pediat, Guangzhou 510080, Guangdong, Peoples R China

来源：

NUCLEIC ACIDS RESEARCH | 2015年 / 43卷 / 21期

关键词：

NOVO DNA METHYLATION; PIWI PROTEINS; DROSOPHILA-MELANOGASTER; CHROMATIN STATE; GENE-EXPRESSION; MOUSE GERMLINE; NONCODING RNA; CANCER-CELLS; 3' TERMINI; IDENTIFICATION;

D O I：

10.1093/nar/gkv954

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and are involved in maintaining genome integrity by silencing transposons. These are also involved in DNA/histone methylation and gene expression regulation in somatic cells of invertebrates. The functions of piRNAs in somatic cells of vertebrates, however, remain elusive. We found that snoRNA-derived and C (C')/D' (D)-box conserved piRNAs are abundant in human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to pre-mRNA intron, which subsequently inhibited the development of Th2 T-lymphocytes. Piwil4 and Ago4 are associated with this piRNA, and this complex further interacts with Trf4-Air2-Mtr4 Polyadenylation (TRAMP) complex, which leads to the decay of targeted pre-mRNA through nuclear exosomes. Taken together, we demonstrate a novel piRNA mechanism in regulating gene expression in highly differentiated somatic cells and a possible novel target for allergy therapeutics.

引用

页码：10474 / 10491

页数：18

共 79 条

[1] Maternally deposited germline piRNAs silence the tirant retrotransposon in somatic cells [J].

Akkouche, Abdou ;

Grentzinger, Thomas ;

Fablet, Marie ;

Armenise, Claudia ;

Burlet, Nelly ;

Braman, Virginie ;

Chambeyron, Severine ;

Vieira, Cristina .

EMBO REPORTS, 2013, 14 (05) :458-464

[2] The small RNA profile during Drosophila melanogaster development [J].

Aravin, AA ;

Lagos-Quintana, M ;

Yalcin, A ;

Zavolan, M ;

Marks, D ;

Snyder, B ;

Gaasterland, T ;

Meyer, J ;

Tuschl, T .

DEVELOPMENTAL CELL, 2003, 5 (02) :337-350

[3] A novel class of small RNAs bind to MILI protein in mouse testes [J].

Aravin, Alexei ;

Gaidatzis, Dimos ;

Pfeffer, Sebastien ;

Lagos-Quintana, Mariana ;

Landgraf, Pablo ;

Iovino, Nicola ;

Morris, Patricia ;

Brownstein, Michael J. ;

Kuramochi-Miyagawa, Satomi ;

Nakano, Toru ;

Chien, Minchen ;

Russo, James J. ;

Ju, Jingyue ;

Sheridan, Robert ;

Sander, Chris ;

Zavolan, Mihaela ;

Tuschl, Thomas .

NATURE, 2006, 442 (7099) :203-207

[4] A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice [J].

Aravin, Alexei A. ;

Sachidanandam, Ravi ;

Bourc'his, Deborah ;

Schaefer, Christopher ;

Pezic, Dubravka ;

Toth, Katalin Fejes ;

Bestor, Timothy ;

Hannon, Gregory J. .

MOLECULAR CELL, 2008, 31 (06) :785-799

[5] piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans [J].

Ashe, Alyson ;

Sapetschnig, Alexandra ;

Weick, Eva-Maria ;

Mitchell, Jacinth ;

Bagijn, Marloes P. ;

Cording, Amy C. ;

Doebley, Anna-Lisa ;

Goldstein, Leonard D. ;

Lehrbach, Nicolas J. ;

Le Pen, Jeremie ;

Pintacuda, Greta ;

Sakaguchi, Aisa ;

Sarkies, Peter ;

Ahmed, Shawn ;

Miska, Eric A. .

CELL, 2012, 150 (01) :88-99

[6] MicroRNA-mediated regulation of T helper cell differentiation and plasticity [J].

Baumjohann, Dirk ;

Ansel, K. Mark .

NATURE REVIEWS IMMUNOLOGY, 2013, 13 (09) :666-678

[7] An Epigenetic Role for Maternally Inherited piRNAs in Transposon Silencing [J].

Brennecke, Julius ;

Malone, Colin D. ;

Aravin, Alexei A. ;

Sachidanandam, Ravi ;

Stark, Alexander ;

Hannon, Gregory J. .

SCIENCE, 2008, 322 (5906) :1387-1392

[8]

Brennecke J, 2007, CELL, V128, P1089, DOI 10.1016/j.cell.2007.01.043

[9] Drosophila PIWI associates with chromatin and interacts directly with HP1a [J].

Brower-Toland, Brent ;

Findley, Seth D. ;

Jiang, Ling ;

Liu, Li ;

Yin, Hang ;

Dus, Monica ;

Zhou, Pei ;

Elgin, Sarah C. R. ;

Lin, Haifan .

GENES & DEVELOPMENT, 2007, 21 (18) :2300-2311

[10] The evolution of gene regulation by transcription factors and microRNAs [J].

Chen, Kevin ;

Rajewsky, Nikolaus .

NATURE REVIEWS GENETICS, 2007, 8 (02) :93-103

← 1 2 3 4 5 6 7 8 →