Electrochemical DNA biosensors for label-free breast cancer gene marker detection

被引：50

作者：

Senel, Mehmet ^{[1
]}

Dervisevic, Muamer ^{[2
]}

Kokkokoglu, Firdevs ^{[3
]}

机构：

[1] Nanoyasam Nanobiotechnol Co, SANKARA Brain & Biotechnol Res Ctr, TR-34320 Istanbul, Turkey

[2] Monash Univ, Monash Inst Pharmaceut Sci, Parkville, Vic 3052, Australia

[3] Dabska 18H, PL-52571 Krakow, Poland

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2019年 / 411卷 / 13期

关键词：

Electrochemical DNA biosensor; Ferrocene; Poly(amidoamine); Dendrimer; Breast cancer; FERROCENE; SENSOR; PAMAM; NANOPARTICLES; CONSTRUCTION; MONOLAYERS; GOLD; ACID;

D O I：

10.1007/s00216-019-01739-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an electrochemical DNA detection strategy based on self-assembled ferrocene-cored poly(amidoamine) dendrimers for the detection of a gene relevant to breast cancer. The chemisorption of three ferrocene-cored poly(amidoamine) generations and hybridization of single-stranded DNA on a Au electrode were studied by cyclic voltammetry and differential pulse voltammetry. The biosensor demonstrated high sensitivity of 0.13 A/(ng/ml) in the detection of the target DNA with a linear range of 1.3-20 nM and a detection limit of 0.38 nM. The DNA biosensor also has high selectivity for the target DNA, showing a clear signal difference from a noncomplementary sequence and a single-base-mismatch sequence, which was used as a model of BRAC1 gene mutation. The results shown are highly motivating for exploring DNA biosensing technology in the diagnosis of breast cancer caused by mutation of the BRAC1 gene.

引用

页码：2925 / 2935

页数：11

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