STARCH AND SODIUM HIPOCHLORITE ON IN VITRO ROOTING OF PINEAPPLE 'GOLD' AND ITS EFFECTS ON ACCLIMATIZATION

被引：3

作者：

Oliveira, Rodrigo Da Silva ^{[1
]}

Pereira, Monique Rodrigues ^{[2
]}

Carvalho, Virginia Silva ^{[1
]}

Lucas, Elizabeth De Fatima

Gravina, Geraldo De Amaral ^{[3
]}

机构：

[1] UENF CCTA LFIT, Campos, RJ, Brazil

[2] UENF CCTA LFIT, Prod Vegetal, Campos, RJ, Brazil

[3] UENF CCTA LEAG, Campos, RJ, Brazil

来源：

REVISTA BRASILEIRA DE FRUTICULTURA | 2015年 / 37卷 / 02期

关键词：

Ananas comosus var. comosus; chemical sterilization; gelling agent; corn starch; cassava starch; in vitro propagation; CHLOROPHYLL FLUORESCENCE; MICROPROPAGATION; STERILIZATION; HYPOCHLORITE;

D O I：

10.1590/0100-2945-120/14

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

This study aimed to evaluate the total or partial replacement of agar by corn starch and cassava starch and physical sterilization in autoclave for chemical sterilization with sodium hypochlorite (NaClO) in development of pineapple 'Gold' plantlets. The gelling agents used were: M1: agar (6.0 g L-1); M2: corn starch (60.0 g L-1); M3: agar (3.0 g L-1) + corn starch (30.0 g L-1) and M4: agar (3.0 g L-1) + cassava starch (30.0 g L-1). The sterilizations were made by autoclaving at 121 degrees C or by using the solutions of NaClO at the concentrations of 0,003% to rinse the glassware and 0,0003% for addition to the culture medium. After one month of in vitro cultivation, it was observed no negative influence of NaClO and gelling agents in the rooting in vitro of shoots for most parameters evaluated. The plantlets derived from in vitro phase were taken to acclimatization in greenhouse for 90 days. During the acclimatization, the plantlets derived from treatments with chemical sterilization did not differ from the ones derived of autoclaved media to any parameter evaluated, whereas the combination agar + corn starch provided inferior results among gelling agents.

引用

页码：273 / 280

页数：8

共 24 条

[1]

BARREIRO NETO M., 2007, RUBI CULTIVAR ABACAX

[2]

BARTHOLOMEW D. P, 2009, PINEAPPLE NEWS, P2

[3] CHLOROPHYLL FLUORESCENCE AS A PROBE OF THE PHOTOSYNTHETIC COMPETENCE OF LEAVES IN THE FIELD - A REVIEW OF CURRENT INSTRUMENTATION [J].